RAPGEF1 Antibody (YA8220)

Cat. No.: HY-P88536: Data Sheet COA
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RAPGEF1 Antibody (YA8220) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RAPGEF1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

RAPGEF1 Antibody (YA8220) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RAPGEF1.

Host

Mouse

Clonality

Monoclonal

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q13905

Immunogen

Full length human recombinant protein of human RAPGEF1 produced in HEK293T cell.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-200
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-250

Sensitivity	Endogenous	Purity	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Guanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. Transduces signals from CRK to activate RAS. Involved in cell branching and adhesion mediated by BCAR1-CRK-RAPGEF1 signaling and activation of RAP1 (PubMed:12432078). Plays a role in the establishment of basal endothelial barrier function. Plays a role in nerve growth factor (NGF)-induced sustained activation of Rap1 and neurite outgrowth

Subcellular Localization：Early endosome

Expression：
Tissue_Specificity: Ubiquitously expressed in adult and fetus. Expression is high in adult skeletal muscle and placenta and in fetal brain and heart. Low levels of expression in adult and fetal liver

Isoforms & Post-Translational Modification：Q13905 has four isomers: Q13905-1: 120548 Da (predicted); Q13905-2: 116141 Da (predicted); Q13905-3: 122735 Da (predicted); Q13905-4: 122287 Da (predicted).
Phosphorylation at Tyr-504 enhances activity as Rap guanine nucleotide exchange factor

Subunit：Interacts with HCK (via SH3-binding sites) (PubMed:14551197)

Synonyms

C3G; GRF2

Documentation

Select Batch:

Data Sheet (231 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)