Copy Link Print Information

NR6A1 Antibody (YA4284)

(Synonyms: RTR; GCNF; NR61; GCNF1)
Customer Review

Based on 1 Customer Validation

NR6A1 Antibody (YA4284) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to NR6A1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, ICC/IF, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
ALL WB FC
  • Experimental Validation Results for NR6A1 Antibody (YA4284)
  • Experimental Validation Results for NR6A1 Antibody (YA4284)
  • Experimental Validation Results for NR6A1 Antibody (YA4284)
  • NR6A1 Antibody (YA4284) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:400 1:200-1:400 1:10000

Product Details

Description

NR6A1 Antibody (YA4284) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to NR6A1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 54 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 54 kDa
Immunogen

Purified recombinant fragment of human NR6A1 (AA: 65-118) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718941

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for NR6A1 Antibody (YA4284)
    Western blot analysis of extracts from HEK293 (lane 2(20μg), HeLa (lane 3(20μg), HUVEC (lane 4(20μg), HepG2 (lane 5(20μg) using NR6A1 Antibody（HY-P84587). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for NR6A1 Antibody (YA4284)
    Western blot analysis of extracts from HUVE-12(lane 1(40μg) )
  • Experimental Validation Results for NR6A1 Antibody (YA4284)
    Flow cytometric analysis of 1X10^6 K562 cells labeling NR6A1 Antibody(HY-P84587

Background

  • Function

    NR6A1 is an Orphan nuclear receptor that binds to a response element containing the sequence 5'-TCAAGGTCA-3'. Acts as a regulator of embryonic stem cell pluripotency by mediating repression of POU5F1/OCT4: binds to the DR0 element within the POU5F1/OCT4 promoter and inhibits POU5F1/OCT4 expression during embryonic stem cell differentiation. Involved in the regulation of gene expression in germ cell development during gametogenesis[1].

  • Subcellular Localization

    Nucleus

  • Expression


    Tissue_specificity:Shows highest expression in the germ cells of the adult testis

  • Subunit

    Homodimer (By similarity). Interacts with UIMC1 (PubMed:12080054)

  • SwissProt ID

    Q15406

  • Gene ID
    2649 [NCBI]
  • Synonyms

    RTR; GCNF; NR61; GCNF1

NR6A1 Antibody (YA4284) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 (lane 2(20μg), HeLa (lane 3(20μg), HUVEC (lane 4(20μg), HepG2 (lane 5(20μg) using NR6A1 Antibody（HY-P84587). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HUVE-12(lane 1(40μg) )

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 K562 cells labeling NR6A1 Antibody(HY-P84587

  • NR6A1 Antibody (YA4284) Product Image
    1/16
  • Experimental Validation Results for NR6A1 Antibody (YA4284)
  • Experimental Validation Results for NR6A1 Antibody (YA4284)
  • Experimental Validation Results for NR6A1 Antibody (YA4284)
  • NR6A1 Antibody (YA4284) Product Image
  • Experimental Validation Results for NR6A1 Antibody (YA4284)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 (lane 2(20μg), HeLa (lane 3(20μg), HUVEC (lane 4(20μg), HepG2 (lane 5(20μg) using NR6A1 Antibody（HY-P84587). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for NR6A1 Antibody (YA4284)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HUVE-12(lane 1(40μg) )
  • Experimental Validation Results for NR6A1 Antibody (YA4284)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 K562 cells labeling NR6A1 Antibody(HY-P84587
  • Experimental Validation Results for NR6A1 Antibody (YA4284)
  • Experimental Validation Results for NR6A1 Antibody (YA4284)
  • Experimental Validation Results for NR6A1 Antibody (YA4284)
  • NR6A1 Antibody (YA4284) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293 (lane 2(20μg), HeLa (lane 3(20μg), HUVEC (lane 4(20μg), HepG2 (lane 5(20μg) using NR6A1 Antibody（HY-P84587). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.

  • NR6A1 Antibody (YA4284) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HUVE-12(lane 1(40μg) )

  • NR6A1 Antibody (YA4284) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 K562 cells labeling NR6A1 Antibody(HY-P84587

  • NR6A1 Antibody (YA4284) Product Image WB
  • NR6A1 Antibody (YA4284) Product Image WB
  • NR6A1 Antibody (YA4284) Product Image FC