Acetyl-Histone H3 (Lys27) Antibody
(Synonyms: H3K27me3; H3 histone; HIST1H3A; Histone cluster 1; H3a)
Based on 1 Customer Validation
Acetyl-Histone H3 (Lys27) Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Acetyl-Histone H3 (Lys27).
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, ChIP
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in TBS (pH7.4), 0.2% BSA, 50% Glycerol, 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ChIP
ChIP: Chromatin Immunoprecipitation
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|---|---|---|---|---|
| Dilution Ratio | 1:50000 | 1:100-500 | 1:2000-1:5000 | Recomend 0.5~2 μg for 25 μg of chromatin. |
Product Details
Acetyl-Histone H3 (Lys27) Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Acetyl-Histone H3 (Lys27).
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Host Rabbit
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 15 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 15 kDa
Synthetic peptide within human Histone H3 aa 20-40 (acetyl Lys27).
Endogenous
affinity purified
Non-conjugated
Acetylated
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in TBS (pH7.4), 0.2% BSA, 50% Glycerol, 0.05% Sodium Azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Acetyl-Histone H3 core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling
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Subcellular Localization
Nucleus; Chromosome
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Subunit
The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with TONSL; CHAF1A; CHAF1B; MCM2 and DNAJC9 (PubMed:33857403). Interacts with NASP; NASP is a histone chaperone that stabilizes and maintains a soluble pool of Histone H3-H4 dimers (PubMed:22195965)
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SwissProt ID
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Synonyms
H3K27me3; H3 histone; HIST1H3A; Histone cluster 1; H3a
Documentation
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