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Glucokinase Antibody (YA2040)

(Synonyms: GCK; Glucokinase; Hexokinase type IV; HK IV; Hexokinase-4; HK4; Hexokinase-D)
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Based on 1 Customer Validation

Glucokinase Antibody (YA2040) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Glucokinase.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Glucokinase Antibody (YA2040)
  • Glucokinase Antibody (YA2040) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Glucokinase Antibody (YA2040) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Glucokinase.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 52 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 52 kDa
Species Reactivity Database

Entrez Gene: 2645 Human

SwissProt: P35557 Human

Immunogen

A synthesized peptide derived from human Glucokinase aa250-465/465.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104406

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Glucokinase Antibody (YA2040)
    Western blot analysis was performed on extracts from BxPC-3 (lane 1, 15 μg), SMMC-7721 (lane 2, 15 μg), HepG2 (lane 3, 15 μg), and SiHa (lane 4, 15 μg) using GCK Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:500 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    Glucokinase is an enzyme that facilitates phosphorylation of glucose to glucose-6-phosphate. Glucokinase occurs in cells in theliver, pancreas, gut, and brain of humans and most other vertebrates. In each of these organs it plays an important role in the regulation of carbohydrate metabolism by acting as a glucose sensor, triggering shifts in metabolism or cell function in response to rising or falling levels of glucose, such as occur after a meal or when fasting. Glucokinase has a lower affinity for glucose than the other hexokinases do, and its activity is localized to a few cell types, leaving the other three hexokinases as more important preparers of glucose for glycolysis and glycogen synthesis for most tissues and organs. Mutations of the gene for this enzyme can cause unusual forms of diabetes or hypoglycemia.

  • Subcellular Localization

    Cytoplasm; Nucleus; Mitochondrion

  • Isoforms & Post-Translational Modification

    P35557 has 3 isomers: P35557-1: 52191 Da (predicted); P35557-2: 52136 Da (predicted); P35557-3: 52035 Da (predicted).

  • Subunit

    Monomer (PubMed:15016359, PubMed:19362831, PubMed:23957911). Interacts with MIDN; the interaction occurs preferentially at low glucose levels and results in inhibition of hexokinase activity (PubMed:24187134). Interacts with GCKR; leading to sequestration in the nucleus (PubMed:10456334)

  • SwissProt ID

    P35557

  • Gene ID
    2645 [NCBI]
  • Synonyms

    GCK; Glucokinase; Hexokinase type IV; HK IV; Hexokinase-4; HK4; Hexokinase-D

  • Research Field

    Signal Transduction

Glucokinase Antibody (YA2040) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from BxPC-3 (lane 1, 15 μg), SMMC-7721 (lane 2, 15 μg), HepG2 (lane 3, 15 μg), and SiHa (lane 4, 15 μg) using GCK Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:500 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Glucokinase Antibody (YA2040) Product Image
    1/16
  • Experimental Validation Results for Glucokinase Antibody (YA2040)
  • Glucokinase Antibody (YA2040) Product Image
  • Experimental Validation Results for Glucokinase Antibody (YA2040)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from BxPC-3 (lane 1, 15 μg), SMMC-7721 (lane 2, 15 μg), HepG2 (lane 3, 15 μg), and SiHa (lane 4, 15 μg) using GCK Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:500 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Glucokinase Antibody (YA2040)
  • Glucokinase Antibody (YA2040) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from BxPC-3 (lane 1, 15 μg), SMMC-7721 (lane 2, 15 μg), HepG2 (lane 3, 15 μg), and SiHa (lane 4, 15 μg) using GCK Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:500 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Glucokinase Antibody (YA2040) Product Image WB