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RelB Antibody (YA098)

(Synonyms: Transcription factor RelB, I-Rel, RELB)
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Based on 1 Customer Validation

RelB Antibody (YA098) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RelB.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for RelB Antibody (YA098)
  • Experimental Validation Results for RelB Antibody (YA098)
  • Experimental Validation Results for RelB Antibody (YA098)
  • RelB Antibody (YA098) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000 1:50-1:200 1:50-1:200 Use at an assay dependent concentration.

Product Details

Description

RelB Antibody (YA098) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RelB.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 70 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 62 kDa
Species Reactivity Database

Entrez Gene: 5971 Human

SwissProt: Q01201 Human

Immunogen

Synthetic peptide corresponding to Human RelB.AA range:1-50.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102416

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for RelB Antibody (YA098)
    Western blot analysis of extracts from Raji (lane 2(20μg), Raji (lane 3(20μg),using RelB Antibody (HY-P80306) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for RelB Antibody (YA098)
    Immunocytochemistry analysis of Hela cells labeling RelB with RelB Antibody (HY-P80306) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with RelB Antibody (HY-P80306) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). Beta tubulin was stained at 1/100 dilution overnight at 4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HY-P8006) was used as the secondary antibody at 1/1,000 dilution.
  • Experimental Validation Results for RelB Antibody (YA098)
    Immunocytochemistry analysis of PANC-1 cells labeling RelB with RelB Antibody (HY-P80306) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with RelB Antibody (HY-P80306)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). Beta tubulin was stained at 1/100 dilution overnight at 4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HY-P8006) was used as the secondary antibody at 1/1,000 dilution.

Background

  • Function

    RelB is a member of the NF-κB transcription factor family and functions as a key regulator of immune homeostasis, antigen-presenting cell activity, and lymphoid tissue organization[4][1]. Mechanistically, RelB is the principal transcriptional component of the non-canonical NF-κB pathway, where NF-κB-inducing kinase (NIK) and IKKα promote processing of p100 into p52, leading to formation and nuclear translocation of RelB:p52 heterodimers that regulate distinct target genes involved in lymphoid organogenesis, lymphocyte trafficking, and immune regulation[1][5]. In dendritic cells, RelB is highly expressed and is required for dendritic cell maturation, antigen presentation, and T-cell activation, highlighting its central role in adaptive immune responses[2]. Disease relevance arises from the broad involvement of non-canonical NF-κB signaling in inflammatory, autoimmune, and malignant disorders, while RelB-dependent transcriptional programs contribute to the regulation of immune response genes and maintenance of immune homeostasis[1][3][6]. Compared with the closely related NF-κB subunit RelA (p65), which predominantly mediates canonical NF-κB signaling through p50:RelA dimers, RelB preferentially forms transcriptionally active complexes with p52 and controls a distinct set of genes associated with developmental and homeostatic immune functions[1][5]. Experimental studies further indicate that RelB can modulate inflammatory gene expression by competing with RelA for κB-binding sites, providing a mechanistic basis for functional divergence among NF-κB family members[6]. For research applications, pharmacological strategies targeting NF-κB signaling, including inhibitors of upstream kinases and nuclear NF-κB activity, are widely used to investigate RelB-regulated pathways in immunity, inflammation, and cancer models[3].

  • Subcellular Localization

    Nucleus; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

  • Expression


    Induction:Up-regulated by mitogens and NUPR1

  • Subunit

    Component of the NF-kappa-B RelB-p50 complex. Component of the NF-kappa-B RelB-p52 complex. Self-associates; the interaction seems to be transient and may prevent degradation allowing for heterodimer formation with p50 or p52. Interacts with NFKB1/p50, NFKB2/p52 and NFKB2/p100. Interacts with NFKBID. Interacts with BMAL1 and the interaction is enhanced in the presence of CLOCK (By similarity)

  • SwissProt ID

    Q01201

  • Gene ID
    5971 [NCBI]
  • Synonyms

    Transcription factor RelB, I-Rel, RELB

  • Research Field

    Cell Biology

[1]. Sun SC. The non-canonical NF-kappaB pathway in immunity and inflammation. Nat Rev Immunol. 2017;17(9):545-558. [Content Brief]

[2]. Shih VFS, et al. Control of RelB during dendritic cell activation integrates canonical and noncanonical NF-kappaB pathways. Nat Immunol. 2012;13(12):1162-1170. [Content Brief]

[3]. Guo Q, et al. NF-κB in biology and targeted therapy: new insights and translational implications. Signal Transduct Target Ther. 2024 Mar 4;9(1):53. [Content Brief]

[4]. Ghosh G, et al. Origin of the Functional Distinctiveness of NF-κB/p52. Front Cell Dev Biol. 2021;9:764164.

[5]. Basak S, et al. Crosstalk via the NF-kB signaling system. Cytokine Growth Factor Rev. 2008;19(3-4):187-197.

[6]. Navarro HI, et al. NF-κB RelB suppresses the inflammatory gene expression programs of dendritic cells by competing with RelA for binding to target gene promoters. Cell Discov. 2025;11(1):1.

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RelB Antibody (YA098) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Raji (lane 2(20μg), Raji (lane 3(20μg),using RelB Antibody (HY-P80306) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling RelB with RelB Antibody (HY-P80306) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with RelB Antibody (HY-P80306) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). Beta tubulin was stained at 1/100 dilution overnight at 4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HY-P8006) was used as the secondary antibody at 1/1,000 dilution.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of PANC-1 cells labeling RelB with RelB Antibody (HY-P80306) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with RelB Antibody (HY-P80306)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). Beta tubulin was stained at 1/100 dilution overnight at 4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HY-P8006) was used as the secondary antibody at 1/1,000 dilution.

  • RelB Antibody (YA098) Product Image
    1/16
  • Experimental Validation Results for RelB Antibody (YA098)
  • Experimental Validation Results for RelB Antibody (YA098)
  • Experimental Validation Results for RelB Antibody (YA098)
  • RelB Antibody (YA098) Product Image
  • Experimental Validation Results for RelB Antibody (YA098)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Raji (lane 2(20μg), Raji (lane 3(20μg),using RelB Antibody (HY-P80306) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for RelB Antibody (YA098)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling RelB with RelB Antibody (HY-P80306) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with RelB Antibody (HY-P80306) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). Beta tubulin was stained at 1/100 dilution overnight at 4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HY-P8006) was used as the secondary antibody at 1/1,000 dilution.
  • Experimental Validation Results for RelB Antibody (YA098)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of PANC-1 cells labeling RelB with RelB Antibody (HY-P80306) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with RelB Antibody (HY-P80306)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). Beta tubulin was stained at 1/100 dilution overnight at 4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HY-P8006) was used as the secondary antibody at 1/1,000 dilution.
  • Experimental Validation Results for RelB Antibody (YA098)
  • Experimental Validation Results for RelB Antibody (YA098)
  • Experimental Validation Results for RelB Antibody (YA098)
  • RelB Antibody (YA098) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Raji (lane 2(20μg), Raji (lane 3(20μg),using RelB Antibody (HY-P80306) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • RelB Antibody (YA098) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling RelB with RelB Antibody (HY-P80306) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with RelB Antibody (HY-P80306) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). Beta tubulin was stained at 1/100 dilution overnight at 4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HY-P8006) was used as the secondary antibody at 1/1,000 dilution.

  • RelB Antibody (YA098) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of PANC-1 cells labeling RelB with RelB Antibody (HY-P80306) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with RelB Antibody (HY-P80306)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). Beta tubulin was stained at 1/100 dilution overnight at 4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HY-P8006) was used as the secondary antibody at 1/1,000 dilution.

  • RelB Antibody (YA098) Product Image WB
  • RelB Antibody (YA098) Product Image ICC
  • RelB Antibody (YA098) Product Image ICC