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RelB Antibody (YA098)

Customer Review

Based on 1 Customer Validation

RelB Antibody (YA098) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RelB.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for RelB Antibody (YA098)
  • Experimental Validation Results for RelB Antibody (YA098)
  • Experimental Validation Results for RelB Antibody (YA098)
  • RelB Antibody (YA098) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000 1:50-1:200 1:50-1:200 Use at an assay dependent concentration.

Product Details

Description

RelB Antibody (YA098) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RelB.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 70 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 62 kDa
Species Reactivity Database

Entrez Gene: 5971 Human

SwissProt: Q01201 Human

Immunogen

Synthetic peptide corresponding to Human RelB.AA range:1-50.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102416

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for RelB Antibody (YA098)
    Western blot analysis of extracts from Raji (lane 2(20μg), Raji (lane 3(20μg),using RelB Antibody (HY-P80306) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for RelB Antibody (YA098)
    Immunocytochemistry analysis of Hela cells labeling RelB with RelB Antibody (HY-P80306) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with RelB Antibody (HY-P80306) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). Beta tubulin was stained at 1/100 dilution overnight at 4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HY-P8006) was used as the secondary antibody at 1/1,000 dilution.
  • Experimental Validation Results for RelB Antibody (YA098)
    Immunocytochemistry analysis of PANC-1 cells labeling RelB with RelB Antibody (HY-P80306) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with RelB Antibody (HY-P80306)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). Beta tubulin was stained at 1/100 dilution overnight at 4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HY-P8006) was used as the secondary antibody at 1/1,000 dilution.

Background

  • Function

    NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric RelB-p50 and RelB-p52 complexes are transcriptional activators. RELB neither associates with DNA nor with RELA/p65 or REL. Stimulates promoter activity in the presence of NFKB2/p49. As a member of the NUPR1/RELB/IER3 survival pathway, may provide pancreatic ductal adenocarcinoma with remarkable resistance to cell stress, such as starvation or gemcitabine treatment. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer in a CRY1/CRY2 independent manner. Increased repression of the heterodimer is seen in the presence of NFKB2/p52. Is required for both T and B lymphocyte maturation and function (PubMed:26385063)

  • Subcellular Localization

    Nucleus; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

  • Expression


    Induction:Up-regulated by mitogens and NUPR1

  • Subunit

    Component of the NF-kappa-B RelB-p50 complex. Component of the NF-kappa-B RelB-p52 complex. Self-associates; the interaction seems to be transient and may prevent degradation allowing for heterodimer formation with p50 or p52. Interacts with NFKB1/p50, NFKB2/p52 and NFKB2/p100. Interacts with NFKBID. Interacts with BMAL1 and the interaction is enhanced in the presence of CLOCK (By similarity)

  • SwissProt ID

    Q01201

  • Gene ID
    5971 [NCBI]
  • Research Field

    Cell Biology

RelB Antibody (YA098) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Raji (lane 2(20μg), Raji (lane 3(20μg),using RelB Antibody (HY-P80306) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling RelB with RelB Antibody (HY-P80306) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with RelB Antibody (HY-P80306) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). Beta tubulin was stained at 1/100 dilution overnight at 4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HY-P8006) was used as the secondary antibody at 1/1,000 dilution.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of PANC-1 cells labeling RelB with RelB Antibody (HY-P80306) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with RelB Antibody (HY-P80306)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). Beta tubulin was stained at 1/100 dilution overnight at 4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HY-P8006) was used as the secondary antibody at 1/1,000 dilution.

  • RelB Antibody (YA098) Product Image
    1/16
  • Experimental Validation Results for RelB Antibody (YA098)
  • Experimental Validation Results for RelB Antibody (YA098)
  • Experimental Validation Results for RelB Antibody (YA098)
  • RelB Antibody (YA098) Product Image
  • Experimental Validation Results for RelB Antibody (YA098)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Raji (lane 2(20μg), Raji (lane 3(20μg),using RelB Antibody (HY-P80306) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for RelB Antibody (YA098)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling RelB with RelB Antibody (HY-P80306) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with RelB Antibody (HY-P80306) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). Beta tubulin was stained at 1/100 dilution overnight at 4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HY-P8006) was used as the secondary antibody at 1/1,000 dilution.
  • Experimental Validation Results for RelB Antibody (YA098)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of PANC-1 cells labeling RelB with RelB Antibody (HY-P80306) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with RelB Antibody (HY-P80306)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). Beta tubulin was stained at 1/100 dilution overnight at 4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HY-P8006) was used as the secondary antibody at 1/1,000 dilution.
  • Experimental Validation Results for RelB Antibody (YA098)
  • Experimental Validation Results for RelB Antibody (YA098)
  • Experimental Validation Results for RelB Antibody (YA098)
  • RelB Antibody (YA098) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Raji (lane 2(20μg), Raji (lane 3(20μg),using RelB Antibody (HY-P80306) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • RelB Antibody (YA098) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling RelB with RelB Antibody (HY-P80306) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with RelB Antibody (HY-P80306) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). Beta tubulin was stained at 1/100 dilution overnight at 4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HY-P8006) was used as the secondary antibody at 1/1,000 dilution.

  • RelB Antibody (YA098) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of PANC-1 cells labeling RelB with RelB Antibody (HY-P80306) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with RelB Antibody (HY-P80306)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). Beta tubulin was stained at 1/100 dilution overnight at 4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HY-P8006) was used as the secondary antibody at 1/1,000 dilution.

  • RelB Antibody (YA098) Product Image WB
  • RelB Antibody (YA098) Product Image ICC
  • RelB Antibody (YA098) Product Image ICC