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MDH2 Antibody (YA3207)(PBS only)

(Synonyms: MDH; MOR1; M-MDH; EIEE51; MGC:3559)

MDH2 Antibody (YA3207) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MDH2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for MDH2 Antibody (YA3207)(PBS only)
  • MDH2 Antibody (YA3207)(PBS only) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

MDH2 Antibody (YA3207) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MDH2.

  • Host Rabbit
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 36 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 36 kDa
Immunogen

A synthetic peptide of human MDH2

Purification

Affinity Purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MDH2 Antibody (YA3207)(PBS only)
    Western blot analysis was performed on extracts from Hela (lane 1, 20 μg), Jurkat (lane 2, 20 μg), K562 (lane 3, 20 μg), HepG2 (lane 4, 20 μg), 293T (lane 5, 20 μg), and A431 (lane 6, 20 μg) using MDH2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit/Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Subcellular Localization

    Mitochondrion matrix

  • Isoforms & Post-Translational Modification

    P40926 has 2 isomers: P40926-1: 35503 Da (predicted); P40926-2: 30875 Da (predicted).
    Acetylation is enhanced by up to 67% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with the appearance of tri- and tetraacetylations. Glucose also increases acetylation by about 60%

  • Subunit

    Homodimer

  • SwissProt ID

    P40926

  • Gene ID
    4191 [NCBI]
  • Synonyms

    MDH; MOR1; M-MDH; EIEE51; MGC:3559

  • Research Field

    Tags & Cell Markers

MDH2 Antibody (YA3207)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 20 μg), Jurkat (lane 2, 20 μg), K562 (lane 3, 20 μg), HepG2 (lane 4, 20 μg), 293T (lane 5, 20 μg), and A431 (lane 6, 20 μg) using MDH2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit/Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • MDH2 Antibody (YA3207)(PBS only) Product Image
    1/16
  • Experimental Validation Results for MDH2 Antibody (YA3207)(PBS only)
  • MDH2 Antibody (YA3207)(PBS only) Product Image
  • Experimental Validation Results for MDH2 Antibody (YA3207)(PBS only)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 20 μg), Jurkat (lane 2, 20 μg), K562 (lane 3, 20 μg), HepG2 (lane 4, 20 μg), 293T (lane 5, 20 μg), and A431 (lane 6, 20 μg) using MDH2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit/Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for MDH2 Antibody (YA3207)(PBS only)
  • MDH2 Antibody (YA3207)(PBS only) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Hela (lane 1, 20 μg), Jurkat (lane 2, 20 μg), K562 (lane 3, 20 μg), HepG2 (lane 4, 20 μg), 293T (lane 5, 20 μg), and A431 (lane 6, 20 μg) using MDH2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit/Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • MDH2 Antibody (YA3207)(PBS only) Product Image WB