2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies
  5. Entactin Antibody (YA2887)

Entactin Antibody (YA2887)

Art. -Nr.: HY-P83142
COA
SDS
User Guide for Antibodies Technical Support

Entactin Antibody (YA2887) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Entactin.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

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  • Beschreibung

Beschreibung

Entactin Antibody (YA2887) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Entactin.
Host

Rabbit
Clonality

Recombinant, Monoclonal
Molekulargewicht
Predicted band size: 136 kDa;
Observed band size: 136 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

Recombinant protein of human Entactin/NID aa844-1245.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:100
Sensitivity Endogenous Reinheit Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Versand

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis of extracts from Hela (lane 2(20μg), A549 (lane 3(20μg) and U87 (lane 4(20μg) using Entactin (HY-P83142) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background
Function：Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions
Subcellular Localization：Secreted, extracellular space, extracellular matrix, basement membrane
Isoforms & Post-Translational Modification：P14543 has 2 isomers: P14543-1: 136377 Da (predicted); P14543-2: 122017 Da (predicted).
N- and O-glycosylated
Subunit：Interacts with FBLN1 (By similarity). Interacts with LGALS3BP (By similarity). Interacts with PLXDC1 (PubMed:16574105). Interacts with SVEP1 (By similarity)
RRID
Database

Entrez Gene: 4811 Human

SwissProt: P14543 Human

OMIM: 131390 Human

Research Field

Signal Transduction
Synonyms
Enactin; Ent; Entactin 1; Entactin; NID 1; NID; Nid1; Nidogen 1; Nidogen
Dokumentation
SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Entactin Antibody (YA2887) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Entactin Antibody (YA2887)
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