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Entactin Antibody (YA2887)

(Synonyms: Enactin; Ent; Entactin 1; Entactin; NID 1; NID; Nid1; Nidogen 1; Nidogen)
Customer Review

Based on 1 Customer Validation

Entactin Antibody (YA2887) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Entactin.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Entactin Antibody (YA2887)
  • Entactin Antibody (YA2887) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

Entactin Antibody (YA2887) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Entactin.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 136 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 136 kDa
Species Reactivity Database

Entrez Gene: 4811 Human

SwissProt: P14543 Human

Immunogen

Recombinant protein of human Entactin/NID aa844-1245.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105257

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Entactin Antibody (YA2887)
    Western blot analysis of extracts from Hela (lane 2(20μg), A549 (lane 3(20μg) and U87 (lane 4(20μg) using Entactin (HY-P83142) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Entactin is a Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions

  • Subcellular Localization

    Secreted, extracellular space, extracellular matrix, basement membrane

  • Isoforms & Post-Translational Modification

    P14543 has 2 isomers: P14543-1: 136377 Da (predicted); P14543-2: 122017 Da (predicted).
    N- and O-glycosylated

  • Subunit

    Interacts with FBLN1 (By similarity). Interacts with LGALS3BP (By similarity). Interacts with PLXDC1 (PubMed:16574105). Interacts with SVEP1 (By similarity)

  • SwissProt ID

    P14543

  • Gene ID
    4811 [NCBI]
  • Synonyms

    Enactin; Ent; Entactin 1; Entactin; NID 1; NID; Nid1; Nidogen 1; Nidogen

  • Research Field

    Signal Transduction

Entactin Antibody (YA2887) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), A549 (lane 3(20μg) and U87 (lane 4(20μg) using Entactin (HY-P83142) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Entactin Antibody (YA2887) Product Image
    1/16
  • Experimental Validation Results for Entactin Antibody (YA2887)
  • Entactin Antibody (YA2887) Product Image
  • Experimental Validation Results for Entactin Antibody (YA2887)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), A549 (lane 3(20μg) and U87 (lane 4(20μg) using Entactin (HY-P83142) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Entactin Antibody (YA2887)
  • Entactin Antibody (YA2887) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg), A549 (lane 3(20μg) and U87 (lane 4(20μg) using Entactin (HY-P83142) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Entactin Antibody (YA2887) Product Image WB