Phospho-CaMK2 alpha (Thr286) Antibody (YA10033)
(Synonyms: Dopamine beta-hydroxylase; Dopamine beta-monooxygenase)Phospho-CaMK2 alpha (Thr286) Antibody (YA10033) is a Rabbit-derived and non-conjugated IgG Monoclonal, Recombinant antibody, targeting to Phospho-CaMK2 alpha (Thr286).
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 50% glycerol, 0.05% BSA, and 0.05% Proclin300.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 |
Product Details
Phospho-CaMK2 alpha (Thr286) Antibody (YA10033) is a Rabbit-derived and non-conjugated IgG Monoclonal, Recombinant antibody, targeting to Phospho-CaMK2 alpha (Thr286).
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Host Rabbit
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Clonality Monoclonal,Recombinant
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Species ReactivityMouse, Rat
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Observed Molecular WeightObserved band size: 50-60 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 54 kDa
Synthetic Phospho-peptide corresponding to residues surrounding T286 of mouse CaMK2 alpha.
Endogenous
affinity purified.
Non-conjugated
Phosphorylated
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 50% glycerol, 0.05% BSA, and 0.05% Proclin300.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
CaMK2 alpha is a Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in various processes, such as synaptic plasticity, neurotransmitter release and long-term potentiation (By similarity). Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission (By similarity). Regulates dendritic spine development (By similarity). Also regulates the migration of developing neurons (By similarity). Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity. Phosphorylates the transcription factor ETS1 in response to calcium signaling, thereby decreasing ETS1 affinity for DNA. In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of STAT1, stimulating the JAK-STAT signaling pathway (By similarity). In response to interferon-beta (IFN-beta) stimulation, stimulates the JAK-STAT signaling pathway (By similarity). In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of PSAT1, inhibiting ferroptosis by promoting GPX4 hydroxylation and stability (By similarity). Acts as a negative regulator of 2-arachidonoylglycerol (2-AG)-mediated synaptic signaling via modulation of DAGLA activity[1][2][3].
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Subcellular Localization
Cytoplasm
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Expression
Tissue_Specificity: Expressed in brain. -
Isoforms & Post-Translational Modification
Phospho-CaMK2 alpha has 2 isoforms, P11798-1: amino acid length is 478, molecular weight is 54115 Da (predicted); P11798-2: amino acid length is 200, molecular weight is 22584 Da (predicted).
Autophosphorylation of Thr-286 following activation by Ca(2+)/calmodulin. Phosphorylation of Thr-286 locks the kinase into an activated state. -
Subunit
There are 4 genes encoding calcium/calmodulin-dependent protein kinase type II chains: CAMK2A, CAMK2B, CAMK2G and CAMK2D.
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SwissProt ID
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Synonyms
Dopamine beta-hydroxylase; Dopamine beta-monooxygenase
Documentation
References
[1]. Tao L, et al. CAMKII and calcineurin regulate the lifespan of Caenorhabditis elegans through the FOXO transcription factor DAF-16. Elife. 2013 Jun 25;2:e00518. [Content Brief]
[2]. Pufall MA, et al. Variable control of Ets-1 DNA binding by multiple phosphates in an unstructured region. Science. 2005 Jul 1;309(5731):142-5. [Content Brief]
[3]. Shonesy BC, et al. CaMKII regulates diacylglycerol lipase-α and striatal endocannabinoid signaling. Nat Neurosci. 2013 Apr;16(4):456-63. [Content Brief]
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