Thioredoxin Antibody (YA5080)
(Synonyms: TXN; TRDX; TRX; TRX1; Thioredoxin; Trx; ATL-derived factor; ADF; Surface-associated sulphydryl protein; SASP)Thioredoxin Antibody (YA5080) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Thioredoxin.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB; ELISA
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Reactivity :
Species independent
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Formulation:
Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|
| Dilution Ratio | 1:500-2000 | 1:1000-5000 |
Product Details
Thioredoxin Antibody (YA5080) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Thioredoxin.
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Host Mouse
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Clonality Monoclonal
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Species ReactivitySpecies independent
Recombinant human Thioredoxin. The exact sequence is proprietary to MCE.
Endogenous
affinity purified by Protein G
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Thioredoxin participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity; ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55)[1][2][3][4][5][6][7].
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Subcellular Localization
Nucleus; Cytoplasm; Secreted
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Expression
Induction:Up-regulated by ionizing radiation -
Isoforms & Post-Translational Modification
P10599 has 2 isomers: P10599-1: 11737 Da (predicted); P10599-2: 9452 Da (predicted).
In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins;In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation -
Subunit
Homodimer; disulfide-linked (PubMed:17260951, PubMed:9369469). Interacts with TXNIP through the redox-active site (PubMed:17260951). Interacts with MAP3K5 and CASP3 (PubMed:15246877). In case of infection, interacts with S.typhimurium protein slrP (PubMed:19690162). Interacts with APEX1; the interaction stimulates the FOS/JUN AP-1 DNA-binding activity in a redox-dependent manner (PubMed:9108029)
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SwissProt ID
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Synonyms
TXN; TRDX; TRX; TRX1; Thioredoxin; Trx; ATL-derived factor; ADF; Surface-associated sulphydryl protein; SASP
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