Nesprin1 Antibody (YA1369)(PBS only)

Cat. No.: HY-P81624A: Data Sheet User Guide for Antibodies
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Nesprin1 Antibody (YA1369) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Nesprin1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Nesprin1 Antibody (YA1369) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Nesprin1.

Host

Rabbit

Molecular Weight

Predicted band size: 1011 kDa;

Observed band size: 112 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q8NF91

Gene ID

23345 [NCBI]

Immunogen

A synthetic peptide of human Nesprin 1

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Multi-isomeric modular protein which forms a linking network between organelles and the actin cytoskeleton to maintain the subcellular spatial organization. As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. May be involved in nucleus-centrosome attachment and nuclear migration in neural progenitors implicating LINC complex association with SUN1/2 and probably association with cytoplasmic dynein-dynactin motor complexes; SYNE1 and SYNE2 may act redundantly. Required for centrosome migration to the apical cell surface during early ciliogenesis. May be involved in nuclear remodeling during sperm head formation in spermatogenesis; a probable SUN3:SYNE1/KASH1 LINC complex may tether spermatid nuclei to posterior cytoskeletal structures such as the manchette

Subcellular Localization：Nucleus outer membrane; Single-pass type IV membrane protein; Cytoplasmic side; Nucleus; Nucleus envelope; Cytoplasm, cytoskeleton; Cytoplasm, myofibril, sarcomere; Golgi apparatus

Expression：
Tissue_specificity:Expressed in HeLa, A431, A172 and HaCaT cells (at protein level) . Widely expressed. Highly expressed in skeletal and smooth muscles, heart, spleen, peripheral blood leukocytes, pancreas, cerebellum, stomach, kidney and placenta. Isoform GSRP-56 is predominantly expressed in heart and skeletal muscle (at protein level)

Isoforms & Post-Translational Modification：Q8NF91 has 12 isomers: Q8NF91-1: 1011086 Da (predicted); Q8NF91-2: 380360 Da (predicted); Q8NF91-3: 112393 Da (predicted); Q8NF91-4: 1005239 Da (predicted); Q8NF91-5: 167250 Da (predicted); Q8NF91-6: 199267 Da (predicted); Q8NF91-7: 642672 Da (predicted); Q8NF91-8: 367873 Da (predicted); Q8NF91-9: 111583 Da (predicted); Q8NF91-10: 349010 Da (predicted); Q8NF91-11: 131185 Da (predicted); Q8NF91-12: 54914 Da (predicted).
The disulfid bond with SUN1 or SUN2 is required for stability of the respective LINC complex under tensile forces

Subunit：Core component of LINC complexes which are composed of inner nuclear membrane SUN domain-containing proteins coupled to outer nuclear membrane KASH domain-containing nesprins. SUN and KASH domain-containing proteins seem to bind each other promiscuously; however, differentially expression of LINC complex constituents can give rise to specific assemblies. At least SUN1/2-containing core LINC complexes are proposed to be hexameric composed of three protomers of each KASH and SUN domain-containing protein. The SUN2:SYNE1/KASH1 LINC complex is a heterohexamer; the homotrimeric cloverleave-like conformation of the SUN domain is a prerequisite for LINC complex formation in which three separate SYNE1/KASH1 peptides bind at the interface of adjacent SUN domains. Self-associates. Interacts with SYNE3. Interacts with SPAG4/SUN4. May interact with MUSK. Interacts with F-actin via its N-terminal domain. Interacts with EMD and LMNA in vitro. Interacts (via KASH domain) with TMEM258 (PubMed:28716842)

Research Field

Tags & Cell Markers

Synonyms

ARCA1; C6orf98; CPG2; EDMD4; Enaptin; Myne-1; MYNE1; Nesp1; Nesprin-1; SCAR8; Syne-1; SYNE1

Documentation

User Guide for Antibodies (1077 KB)