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MATK Antibody (YA4016)

(Synonyms: CHK; CTK)
Customer Review

Based on 1 Customer Validation

MATK Antibody (YA4016) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MATK.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in ascitic fluid containing 0.03% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for MATK Antibody (YA4016)
  • MATK Antibody (YA4016) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:400 1:10000

Product Details

Description

MATK Antibody (YA4016) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MATK.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 56 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 56 kDa
Immunogen

Purified recombinant fragment of human MATK aa 50-196.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718881

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in ascitic fluid containing 0.03% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MATK Antibody (YA4016)
    Western blot analysis of extracts from Jurkat (lane 2(20μg), HepG2 (lane 3(20μg), K562 (lane 4(20μg), Ramos (lane 5(20μg) using MATK Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    MATK is a Could play a significant role in the signal transduction of hematopoietic cells. May regulate tyrosine kinase activity of SRC-family members in brain by specifically phosphorylating their C-terminal regulatory tyrosine residue which acts as a negative regulatory site. It may play an inhibitory role in the control of T-cell proliferation

  • Subcellular Localization

    Cytoplasm; Membrane

  • Expression


    Tissue_specificity:It is expressed in multiple myeloid cell lines and detected in the brain and lungs.

  • Isoforms & Post-Translational Modification

    P42679 has 3 isomers: P42679-1: 56469 Da (predicted); P42679-2: 56798 Da (predicted); P42679-3: 51899 Da (predicted).

  • Subunit

    Interacts with KIT

  • SwissProt ID

    P42679

  • Gene ID
    4145 [NCBI]
  • Synonyms

    CHK; CTK

MATK Antibody (YA4016) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat (lane 2(20μg), HepG2 (lane 3(20μg), K562 (lane 4(20μg), Ramos (lane 5(20μg) using MATK Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • MATK Antibody (YA4016) Product Image
    1/16
  • Experimental Validation Results for MATK Antibody (YA4016)
  • MATK Antibody (YA4016) Product Image
  • Experimental Validation Results for MATK Antibody (YA4016)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat (lane 2(20μg), HepG2 (lane 3(20μg), K562 (lane 4(20μg), Ramos (lane 5(20μg) using MATK Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for MATK Antibody (YA4016)
  • MATK Antibody (YA4016) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Jurkat (lane 2(20μg), HepG2 (lane 3(20μg), K562 (lane 4(20μg), Ramos (lane 5(20μg) using MATK Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • MATK Antibody (YA4016) Product Image WB