LILRB2 Antibody (YA4368)(PBS only)

Cat. No.: HY-P84671A: Data Sheet User Guide for Antibodies
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LILRB2 Antibody (YA4368) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to LILRB2.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

LILRB2 Antibody (YA4368) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to LILRB2.

Host

Mouse

Molecular Weight

Predicted band size: 65 kDa;

Observed band size: 60-65 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q8N423

Gene ID

10288 [NCBI]

Immunogen

Purified recombinant fragment of human LILRB2 (AA: 51-184) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG2a

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles (PubMed:11169396, PubMed:12853576, PubMed:16455647, PubMed:20448110, PubMed:27859042). Involved in the down-regulation of the immune response and the development of tolerance. Recognizes HLA-G in complex with B2M/beta-2 microglobulin and a nonamer self-peptide (peptide-bound HLA-G-B2M) triggering differentiation of type 1 regulatory T cells and myeloid-derived suppressor cells, both of which actively maintain maternal-fetal tolerance (PubMed:16455647, PubMed:20448110, PubMed:27859042). Competes with CD8A for binding to class I MHC antigens. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions (PubMed:11875462, PubMed:12853576, PubMed:9548455, PubMed:9842885)

Subcellular Localization：Cell membrane; Single-pass type I membrane protein

Expression：
Tissue_specificity:Expressed in monocytes and at lower levels in myeloid and plasmacytoid dendritic cells. Expressed in tolerogenic IL10-producing dendritic cells (PubMed:20448110) . Expressed in myeloid-derived suppressor cells during pregnancy (PubMed:27859042) . Detected at low levels in natural killer (NK) cells. Expressed in B cells

Induction:Induced on monocyte-derived macrophages by S.typhimurium infection

Isoforms & Post-Translational Modification：Q8N423 has 4 isomers: Q8N423-2: 65005 Da (predicted); Q8N423-1: 65076 Da (predicted); Q8N423-3: 55495 Da (predicted); Q8N423-4: 51977 Da (predicted).
Phosphorylated on tyrosine residues. Dephosphorylated by PTPN6

Subunit：Binds PTPN6 when phosphorylated. Binds FCGR1A. Interacts with peptide-bound HLA-G-B2M; this interaction is direct (PubMed:16455647). Interacts with peptide-bound HLA-F-B2M; this interaction is direct (PubMed:11169396)

Synonyms

ILT4; LIR2; CD85D; ILT-4; LIR-2; MIR10; MIR-10

Documentation

User Guide for Antibodies (1077 KB)