Lck Antibody (YA4947)
(Synonyms: LCK; Tyrosine-protein kinase Lck; Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell-specific protein-tyrosine kinase; Protein YT16; Proto-oncogene Lck; T cell-specific protein-tyrosine kinase; p56-LCK)Lck Antibody (YA4947) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to Lck.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB; IHC-P; IHC-F; ICC/IF; IF-Tissue; IP; FC
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Reactivity :
Human
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Formulation:
Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IHC-F
IHC-F: Immunohistochemistry-Frozen
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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IP
IP: Immunoprecipitation
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FC
FC: Flow Cytometry
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|---|---|---|---|---|---|---|---|
| Dilution Ratio | 1:500-2000 | 1:100-200 | 1:100-200 | 1:50-200 | 1:50-200 | 1:20-50 | 1:50-100 |
Product Details
Lck Antibody (YA4947) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to Lck.
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Host Rabbit
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Clonality Monoclonal,Recombinant
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 58 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 58 kDa
A synthesized peptide derived from human LCK. The exact sequence is proprietary to MCE.
Endogenous
affinity purified by Protein A
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Lck (lymphocyte-specific protein tyrosine kinase) is a Src-family non-receptor tyrosine kinase that functions as a central initiator of T-cell activation and antigen receptor signaling through phosphorylation-dependent intracellular signal transduction mechanisms[1][2]. Mechanistically, Lck operates at the proximal stage of the T-cell receptor (TCR) signaling pathway, where it phosphorylates immunoreceptor tyrosine-based activation motifs (ITAMs) and promotes downstream signaling events required for T-cell activation, differentiation, and immune responses[2][3]. Genetic studies demonstrated that loss of functional Lck disrupts TCR-mediated signaling and impairs cellular responsiveness, establishing its essential role in lymphocyte biology and experimental models of immune deficiency[4]. Disease relevance is supported by evidence linking LCK deficiency to severe immunological dysfunction, while dysregulated Lck signaling has also been investigated in hematologic malignancies and other immune-associated disorders[1][4]. Compared with related Src-family kinases, particularly Fyn, Lck has a more dominant role in initiating proximal TCR signaling, whereas Fyn contributes to overlapping but nonessential signaling functions in many T-cell activation contexts[3][5]. Structurally, Lck contains SH2, SH3, and catalytic kinase domains that coordinate activation-state regulation through phosphorylation-dependent conformational changes[2]. For experimental applications, Lck remains a widely used target for kinase inhibition studies and mechanistic analyses of T-cell signaling networks, making it a valuable tool for immune signaling research and therapeutic target validation[6].
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Subcellular Localization
Cell membrane; Lipid-anchor; Cytoplasmic side; Cytoplasm, cytosol
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Expression
Tissue_specificity:Specifically expressed in lymphocytes -
Isoforms & Post-Translational Modification
P06239 has 3 isomers: P06239-1: 58001 Da (predicted); P06239-2: 40866 Da (predicted); P06239-3: 61190 Da (predicted).
Autophosphorylated on Tyr-394, increasing enzymatic activity, this site is dephosphorylated by PTN22. Phosphorylated on Tyr-505 by CSK, decreasing activity. Dephosphorylated by PTPRC/CD45. Dephosphorylation at Tyr-394 by PTPN2 negatively regulates T-cell receptor signaling. Dephosphorylation at Tyr-394 by DUSP22 negatively regulates T-cell receptor signaling (PubMed:24714587, PubMed:38225265);Myristoylation is required prior to palmitoylation;Palmitoylation regulates association with the plasma membrane and could be mediated by ZDHHC2;'Lys-63'-linked ubiquitinated at Lys-99 and Lys-276 by UBR2; this modification is required for autophosphorylation at Tyr-394 -
Subunit
Binds to the cytoplasmic domain of cell surface receptors, such as AXL, CD2, CD4, CD5, CD8, CD44, CD45 and CD122. Also binds to effector molecules, such as PI4K, VAV1, RASA1, FYB1 and to other protein kinases including CDK1, RAF1, ZAP70 and SYK. Binds to phosphatidylinositol 3'-kinase (PI3K) from T-lymphocytes through its SH3 domain and to the tyrosine phosphorylated form of KHDRBS1/p70 through its SH2 domain. This interaction inhibits its tyrosine-kinase activity. Interacts with SQSTM1. Interacts with phosphorylated LIME1. Interacts with CBLB and PTPRH. Interacts with RUNX3. Forms a signaling complex with EPHA1, PTK2B and PI3-KINASE; upon activation by EFNA1 which may regulate T-lymphocyte migration. Associates with ZAP70 and RHOH; these interactions allow LCK-mediated RHOH and CD3 subunit phosphorylation in the presence of functional ZAP70. Interacts with UNC119; this interaction plays a crucial role in activation of LCK. Interacts with CEACAM1 (via cytoplasmic domain); mediates CEACAM1 phosphorylation resulting in PTPN6 recruitment that dephosphorylates TCR stimulation-induced CD247 and ZAP70 (PubMed:18424730). Interacts with CD160. Interacts with CD48 (PubMed:12007789)
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SwissProt ID
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Synonyms
LCK; Tyrosine-protein kinase Lck; Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell-specific protein-tyrosine kinase; Protein YT16; Proto-oncogene Lck; T cell-specific protein-tyrosine kinase; p56-LCK
Documentation
[1]. Uniprotkb.
[2]. Rossy J, et al. How does the kinase Lck phosphorylate the T cell receptor? Spatial organization as a regulatory mechanism. Front Immunol. 2012 Jun 19;3:167. [Content Brief]
[3]. Lck gene information from NCBI.
[4]. Straus DB, et al. Genetic evidence for the involvement of the lck tyrosine kinase in signal transduction through the T cell antigen receptor. Cell. 1992 Aug 21;70(4):585-93. [Content Brief]
[5]. Wikipedia.
[6]. Elkamhawy A, et al. New horizons in drug discovery of lymphocyte-specific protein tyrosine kinase (Lck) inhibitors: a decade review (2011-2021) focussing on structure-activity relationship (SAR) and docking insights. J Enzyme Inhib Med Chem. 2021 Dec;36(1):1574-1602. [Content Brief]
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