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CaMKII gamma Antibody (YA4248)(PBS only)

(Synonyms: CAMK; CAMKG; CAMK-II)

CaMKII gamma Antibody (YA4248) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CAMK2 gamma.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, FC, ELISA

  • Reactivity :

    Human, Mouse, Rat, Monkey, Rabbit

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)(PBS only)
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)(PBS only)
  • CaMKII gamma Antibody (YA4248)(PBS only) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:100-1:500 1:200-1:400 1:10000

Product Details

Description

CaMKII gamma Antibody (YA4248) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CAMK2 gamma.

  • Host Mouse
  • Species Reactivity
    Human, Mouse, Rat, Monkey, Rabbit
  • Observed Molecular Weight
    Observed band size: 63 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 63 kDa
Immunogen

Purified recombinant fragment of human CAMK2G (AA: 322-481) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)(PBS only)
    Western blot analysis of extracts from Jurkat (lane 2(20μg), HepG2 (lane 3(20μg), C2C12 (lane 4(20μg), C6 (lane 5(20μg), using CAMK2 gamma Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)(PBS only)
    Flow cytometric analysis of 1X10^6 Jurkat cells labeling CAMK2 gamma Antibody (HY-P84551, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    CAMK2 gamma is a Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in sarcoplasmic reticulum Ca(2+) transport in skeletal muscle and may function in dendritic spine and synapse formation and neuronal plasticity. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR tH2O2gh phosphorylation of the ryanodine receptor-coupling factor triadin. In the central nervous system, it is involved in the regulation of neurite formation and arborization. It may participate in the promotion of dendritic spine and synapse formation and maintenance of synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of STAT1, stimulating the JAK-STAT signaling pathway[1][2].

  • Subcellular Localization

    Sarcoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side

  • Expression


    Tissue_specificity:Expressed in skeletal muscle

    Induction:Activity is induced in skeletal muscle during exercise

  • Subunit

    CAMK2 is composed of 4 different chains: alpha (CAMK2A), beta (CAMK2B), gamma (CAMK2G), and delta (CAMK2D).

  • SwissProt ID

    Q13555

  • Gene ID
    818 [NCBI]
  • Synonyms

    CAMK; CAMKG; CAMK-II

CaMKII gamma Antibody (YA4248)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat (lane 2(20μg), HepG2 (lane 3(20μg), C2C12 (lane 4(20μg), C6 (lane 5(20μg), using CAMK2 gamma Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Jurkat cells labeling CAMK2 gamma Antibody (HY-P84551, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • CaMKII gamma Antibody (YA4248)(PBS only) Product Image
    1/16
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)(PBS only)
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)(PBS only)
  • CaMKII gamma Antibody (YA4248)(PBS only) Product Image
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)(PBS only)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat (lane 2(20μg), HepG2 (lane 3(20μg), C2C12 (lane 4(20μg), C6 (lane 5(20μg), using CAMK2 gamma Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)(PBS only)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Jurkat cells labeling CAMK2 gamma Antibody (HY-P84551, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)(PBS only)
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)(PBS only)
  • CaMKII gamma Antibody (YA4248)(PBS only) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Jurkat (lane 2(20μg), HepG2 (lane 3(20μg), C2C12 (lane 4(20μg), C6 (lane 5(20μg), using CAMK2 gamma Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • CaMKII gamma Antibody (YA4248)(PBS only) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 Jurkat cells labeling CAMK2 gamma Antibody (HY-P84551, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • CaMKII gamma Antibody (YA4248)(PBS only) Product Image WB
  • CaMKII gamma Antibody (YA4248)(PBS only) Product Image FC