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SAE1 Antibody (YA1847)

(Synonyms: SAE1; AOS1; SUA1; UBLE1A; SUMO-activating enzyme subunit 1; Ubiquitin-like 1-activating enzyme E1A)
Customer Review

Based on 1 Customer Validation

SAE1 Antibody (YA1847) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SAE1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP, FC

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in rabbit IgG in 50 mM Tris-Glycine(pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for SAE1 Antibody (YA1847)
  • Experimental Validation Results for SAE1 Antibody (YA1847)
  • SAE1 Antibody (YA1847) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:200 1:50 1:50-1:100

Product Details

Description

SAE1 Antibody (YA1847) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SAE1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 38 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 38 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human SAE1 aa201-248.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104217

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in rabbit IgG in 50 mM Tris-Glycine(pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for SAE1 Antibody (YA1847)
    Western blot analysis was performed on extracts from HeLa (lane 1, 20 μg), Jurkat (lane 2, 20 μg), K562 (lane 3, 20 μg), and A549 (lane 4, 20 μg) using SAE1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit/Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for SAE1 Antibody (YA1847)
    Flow cytometric analysis of 1X106 HeLa cells labeling SAE1 Antibody (HY-P82102, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    SAE1 is a The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2

  • Subcellular Localization

    Nucleus

  • Expression


    Tissue_specificity:Expression level increases during S phase and drops in G2 phase (at protein level)

  • Isoforms & Post-Translational Modification

    Q9UBE0 has 3 isomers: Q9UBE0-1: 38450 Da (predicted); Q9UBE0-2: 29811 Da (predicted); Q9UBE0-3: 33063 Da (predicted).

  • Subunit

    Heterodimer of SAE1 and UBA2/SAE2. The heterodimer corresponds to the two domains that are encoded on a single polypeptide chain in ubiquitin-activating enzyme E1. Interacts with UBE2I

  • SwissProt ID

    Q9UBE0

  • Gene ID
    10055 [NCBI]
  • Synonyms

    SAE1; AOS1; SUA1; UBLE1A; SUMO-activating enzyme subunit 1; Ubiquitin-like 1-activating enzyme E1A

  • Research Field

    Cell Biology

SAE1 Antibody (YA1847) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from HeLa (lane 1, 20 μg), Jurkat (lane 2, 20 μg), K562 (lane 3, 20 μg), and A549 (lane 4, 20 μg) using SAE1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit/Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling SAE1 Antibody (HY-P82102, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • SAE1 Antibody (YA1847) Product Image
    1/16
  • Experimental Validation Results for SAE1 Antibody (YA1847)
  • Experimental Validation Results for SAE1 Antibody (YA1847)
  • SAE1 Antibody (YA1847) Product Image
  • Experimental Validation Results for SAE1 Antibody (YA1847)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from HeLa (lane 1, 20 μg), Jurkat (lane 2, 20 μg), K562 (lane 3, 20 μg), and A549 (lane 4, 20 μg) using SAE1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit/Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for SAE1 Antibody (YA1847)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling SAE1 Antibody (HY-P82102, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for SAE1 Antibody (YA1847)
  • Experimental Validation Results for SAE1 Antibody (YA1847)
  • SAE1 Antibody (YA1847) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from HeLa (lane 1, 20 μg), Jurkat (lane 2, 20 μg), K562 (lane 3, 20 μg), and A549 (lane 4, 20 μg) using SAE1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit/Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • SAE1 Antibody (YA1847) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 HeLa cells labeling SAE1 Antibody (HY-P82102, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • SAE1 Antibody (YA1847) Product Image WB
  • SAE1 Antibody (YA1847) Product Image FC