ABCG1 Antibody (YA1841)(PBS only)

Cat. No.: HY-P82096A: Data Sheet User Guide for Antibodies
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ABCG1 Antibody (YA1841) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ABCG1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

ABCG1 Antibody (YA1841) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ABCG1.

Host

Rabbit

Molecular Weight

Predicted band size: 76 kDa;

Observed band size: 100 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P45844

Gene ID

9619 [NCBI]

Immunogen

A synthetic peptide of human ABCG1

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Catalyzes the efflux of phospholipids such as sphingomyelin, cholesterol and its oxygenated derivatives like 7beta-hydroxycholesterol and this transport is coupled to hydrolysis of ATP (PubMed:17408620, PubMed:24576892). The lipid efflux is ALB-dependent (PubMed:16702602). Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages. Prevents cell death by transporting cytotoxic 7beta-hydroxycholesterol (PubMed:17408620)

Subcellular Localization：Endoplasmic reticulum membrane; Multi-pass membrane protein; Golgi apparatus membrane; Multi-pass membrane protein; Cell membrane

Expression：
Tissue_specificity:Expressed in several tissues. Expressed in macrophages; expression is increased in macrophages from with Tangier disease

Induction:Strongly induced in monocyte-derived macrophages during cholesterol influx. Conversely, mRNA and protein expression are suppressed by lipid efflux. Induction is mediated by the liver X receptor/retinoid X receptor (LXR/RXR) pathway. Not induced by bacterial lipopolysaccharides (LPS) . Repressed by ZNF202

Isoforms & Post-Translational Modification：P45844 has 8 isomers: P45844-1: 75592 Da (predicted); P45844-2: 75380 Da (predicted); P45844-3: 74611 Da (predicted); P45844-4: 74155 Da (predicted); P45844-5: 74032 Da (predicted); P45844-6: 73737 Da (predicted); P45844-7: 71960 Da (predicted); P45844-8: 86628 Da (predicted).
Palmitoylation at Cys-315 seems important for trafficking from the endoplasmic reticulum

Subunit：Homodimer; disulfide-linked (PubMed:16702602). Homooligomer (PubMed:16702602). May form heterodimers with several heterologous partners of the ABCG subfamily. Forms heterodimers with ABCG4 (PubMed:27228027). Interacts with CAV1; this interaction regulates ABCG1-mediated cholesterol efflux (PubMed:24576892)

Research Field

Neuroscience

Synonyms

ATP-binding cassette transporter 8; White protein homolog; ABC8; WHT1; ABCG1; ABC transporter 8; WHITE1

Documentation

User Guide for Antibodies (1077 KB)