ABCG1 Antibody (YA1841)(PBS only)
(Synonyms: ATP-binding cassette transporter 8; White protein homolog; ABC8; WHT1; ABCG1; ABC transporter 8; WHITE1)ABCG1 Antibody (YA1841) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ABCG1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB
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Reactivity :
Human
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
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Applications
| Application |
WB
WB: Western Blot
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|---|---|
| Dilution Ratio | 1:500-1:1000 |
Product Details
ABCG1 Antibody (YA1841) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ABCG1.
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Host Rabbit
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 100 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 76 kDa
A synthetic peptide of human ABCG1
Affinity Purified
Non-conjugated
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using ABCG1 Antibody (HY-P82096) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Background
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Function
ABCG1 catalyzes the efflux of phospholipids such as sphingomyelin, cholesterol and its oxygenated derivatives like 7beta-hydroxycholesterol and this transport is coupled to hydrolysis of ATP. The lipid efflux is ALB-dependent. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages. Prevents cell death by transporting cytotoxic 7beta-hydroxycholesterol[1][2][3].
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Subcellular Localization
Endoplasmic reticulum membrane; Multi-pass membrane protein; Golgi apparatus membrane; Multi-pass membrane protein; Cell membrane
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Expression
Tissue_specificity:Expressed in several tissues. Expressed in macrophages; expression is increased in macrophages from with Tangier disease
Induction:Strongly induced in monocyte-derived macrophages during cholesterol influx. Conversely, mRNA and protein expression are suppressed by lipid efflux. Induction is mediated by the liver X receptor/retinoid X receptor (LXR/RXR) pathway. Not induced by bacterial lipopolysaccharides (LPS) . Repressed by ZNF202 -
Isoforms & Post-Translational Modification
P45844 has 8 isomers: P45844-1: 75592 Da (predicted); P45844-2: 75380 Da (predicted); P45844-3: 74611 Da (predicted); P45844-4: 74155 Da (predicted); P45844-5: 74032 Da (predicted); P45844-6: 73737 Da (predicted); P45844-7: 71960 Da (predicted); P45844-8: 86628 Da (predicted).
Palmitoylation at Cys-315 seems important for trafficking from the endoplasmic reticulum -
Subunit
Homodimer; disulfide-linked (PubMed:16702602). Homooligomer (PubMed:16702602). May form heterodimers with several heterologous partners of the ABCG subfamily. Forms heterodimers with ABCG4 (PubMed:27228027). Interacts with CAV1; this interaction regulates ABCG1-mediated cholesterol efflux (PubMed:24576892)
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SwissProt ID
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Synonyms
ATP-binding cassette transporter 8; White protein homolog; ABC8; WHT1; ABCG1; ABC transporter 8; WHITE1
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Research Field
Neuroscience
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