Histone H2A.Z Antibody (YA7381)
Based on 1 Customer Validation
Histone H2A.Z Antibody (YA7381) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Histone H2A.Z.
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Host:
Rabbit
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Isotype:
IgG,Kappa
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Application:
WB, IHC-P, ICC/IF, IP, ELISA, Cut&Tag
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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IP
IP: Immunoprecipitation
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CHIP
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Cut&Tag
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|---|---|---|---|---|---|---|---|
| Dilution Ratio | 1:200-1:1000 | 1:2000-1:10000 | 1:200-1:1000 | 1:5000-1:20000 | 1:50-1:200 | 1:50-1:100 | 1:50-1:100 |
Product Details
Histone H2A.Z Antibody (YA7381) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Histone H2A.Z.
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Host Rabbit
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Clonality Monoclonal,Recombinant
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 14 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 14 kDa
The exact sequence is proprietary to MCE.
Endogenous
Protein A affinity purified
Non-conjugated
Unmodified
IgG,Kappa
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from U-87MG (lane 1(20μg)) 、HEK293 (lane 2(20μg)) 、Mouse testis (lane 3(20μg)) 、Rat testis (lane 4(20μg)) and Rat lung (lane 5(20μg)) using Histone H2A.Z Antibody (HY-P87696) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
Background
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Function
Histone H2A.Z is a Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division
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Subcellular Localization
Nucleus,Chromosome
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Isoforms & Post-Translational Modification
P0C0S5: 128 amino acids, molecular weight 13553 Da.
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Subunit
The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers
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SwissProt ID
Documentation
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