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  3. Enzymes & Regulators
  4. Transferases (EC 2)
  5. PARP Protein, Mouse (sf9, His)

PARP Protein, Mouse (sf9, His)

Cat. No.: HY-P73337
Handling Instructions

PARP, a poly-ADP-ribosyltransferase, catalyzes poly-ADP-ribosylation, crucial for DNA repair. This protein irreversibly binds to DNA breaks, disrupting repair processes and triggering DNA damage-induced apoptosis. PARP Protein, Mouse (sf9, His) is the recombinant mouse-derived PARP protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of PARP Protein, Mouse (sf9, His) is 1014 a.a., with molecular weight of ~75 kDa.

For research use only. We do not sell to patients.

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Description

PARP, a poly-ADP-ribosyltransferase, catalyzes poly-ADP-ribosylation, crucial for DNA repair. This protein irreversibly binds to DNA breaks, disrupting repair processes and triggering DNA damage-induced apoptosis. PARP Protein, Mouse (sf9, His) is the recombinant mouse-derived PARP protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of PARP Protein, Mouse (sf9, His) is 1014 a.a., with molecular weight of ~75 kDa.
Background

PARP protein, a poly-ADP-ribosyltransferase, is responsible for catalyzing the poly-ADP-ribosylation of proteins and serves as a critical player in DNA repair mechanisms. Specifically, this protein binds irreversibly to DNA breaks, hindering the process of DNA repair and ultimately leading to DNA damage-induced apoptosis.
Species

Mouse
Source

Sf9 insect cells

Tag

N-His
Accession

Q921K2 (M1-W1014)
Gene ID
Molecular Construction
N-term
His
PARP (M1-W1014)
Accession # Q921K2
C-term
Synonyms
ADPRT 1; ADPRT; PARP apoptosis; PARP; PPOL
Molecular Weight

Approximately 75 kDa
Purity

Greater than 85% as determined by reducing SDS-PAGE
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

PARP Protein, Mouse (sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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