Hyaluronidase, Ovine testes 

Hyaluronidase, Ovine testes is an endoglycosidase. Hyaluronidase, Ovine testes specifically degrades Hyaluronic acid (HY-B0633A) and Chondroitin sulfate (HY-B2162) by hydrolyzing β-glycosidic bonds in acidic mucopolysaccharides. Hyaluronidase, Ovine testes disperses follicular cells during fertilization by breaking down the hyaluronic acid-rich cumulus. Hyaluronidase, Ovine testes can be used in the study of fertility-related diseases^[1][2][3].

Hyaluronidase inhibition test
Mix 10 μL of 8 mg/mL Hyaluronidase with the sample in a buffer solution (0.1 M acetate buffer, pH 3.6) and add it to a test tube. Incubate at 37 °C for 20 minutes.
(2) Add 20 μL of 12.5 mM calcium chloride to the mixture and incubate for another 20 minutes at 37°C.
(3) The activated Ca2+ mixture was treated with 50 μL of 2.4 μg/mL hyaluronic acid in the buffer and further incubated at 37°C for 40 minutes.
(4) Add 2 μL of 0.4 N sodium hydroxide and 20 μL of 0.4 N potassium tetraborate tetrahydrate, and then incubate in a water bath at 100°C for 3 minutes.
After cooling to room temperature, add 600 μL of DMAB solution to the mixture and incubate at 37°C for 20 minutes.
(6) Measure the absorbance at 585 nm using an microplate reader.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

37326-33-3

Solid

Off-white to yellow

[Hyaluronidase, Ovine testes]

3.2.1.35

55000 (average)

≥400 U/mg protein

One unit is defined as the amount of enzyme that can cause a change in A600 of 0.330 per minute at pH 5.35, 37°C in a 2.0 mL reaction mixture (45 min assay).

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Krishnapillai A M, et al. Characterisation of Norway lobster (Nephrops norvegicus) hyaluronidase and comparison with sheep and bovine testicular hyaluronidase. Food chemistry, 1999, 65(4): 515-521.

  [2]. SAKAI Y, et al. Immunohistochemical demonstration of hyaluronidase in the sheep testis. Acta Histochemica et Cytochemica, 1979, 12(2): 141-150.

  [3]. Modarressi SM, et al. Enhancing hyaluronidase enzyme activity: Insights from advancement in bovine and ovine testicular hyaluronidase purification. J Chromatogr B Analyt Technol Biomed Life Sci. 2024 Feb 15;1234:124031.  [Content Brief]