Neomycin sulfate

Name: Neomycin sulfate
Brand: MedChemExpress
SKU: HY-B0470
Rating: 5.0 (68 reviews)

Cat. No.: HY-B0470 Purity: 99.86%: Data Sheet
COA

SDS
Handling Instructions
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Neomycin sulfate, an aminoglycoside antibiotic, exerts antibacterial activity through irreversible binding of the nuclear 30S ribosomal subunit, thereby blocking bacterial protein synthesis. Neomycin sulfate is a known phospholipase C (PLC) inhibitor. Neomycin sulfate potently inhibits both the nuclear translocation of angiogenin and angiogenin-induced cell proliferation and angiogenesis. Neomycin sulfate inhibits IP3-mediated Ca²⁺ release, MgATP-dependent Ca²⁺ uptake, and electrical excitation-evoked skeletal muscle Ca²⁺ transients. Neomycin sulfate depletes gut microbiota in specific mouse models, causes hearing impairment, and kidney damage with prolonged exposure. Neomycin sulfate can be used for the research of cancer.

For research use only. We do not sell to patients.

CAS No. : 1405-10-3

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	In-stock	Estimated Time of Arrival: December 31
50 g	Get quote

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Customer Review

Based on 68 publication(s) in Google Scholar

Other Forms of Neomycin sulfate:

Framycetin In-stock
Neomycin sulfate (Standard) In-stock
Neomycin Get quote

68 Publications Citing Use of MCE Neomycin sulfate

In Vivo Efficacy Study

PK/PD Analysis

Others

Bio/Physico-chemical Assay

: Neomycin sulfate purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Apr;12(16):e2415041. [Abstract]; ABR thresholds in each group. The statistical differences were analyzed between Neomycin (100 mg/kg once daily for 14 days, ip) + PO group and Neomycin group.

: Neomycin sulfate purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Apr;12(16):e2415041. [Abstract]; Effects of different levels of UA on HCs in the apical, middle, and basal turns after Neomycin (0.5 mM) treatment for 24 h.

: Neomycin sulfate purchased from MedChemExpress. Usage Cited in: Gut Microbes. 2023 Dec;15(2):2282790. [Abstract]; Depletion of CD8+ T cells in ABX-treated B16-F0 tumor-bearing mice dampened the antitumor effect of butyrate combined with anti-PD-1 (Figure 4b–e). Decreased serum IFN-γ and TNF-α production during the depletion of CD8+ T cells was also observed. ABX：an antibiotic cocktail of 0.5 g/L Vancomycin, 1 g/L Metronidazole, 1 g/L Neomycin and 1 g/L Ampicillin.

: Neomycin sulfate purchased from MedChemExpress. Usage Cited in: Gut Microbes. 2023 Dec;15(2):2249143. [Abstract]; Targeted metabolomics analysis of gut microbial metabolites (AA, PA, and BA) in colon contents (feces) and serum from mice with or without ABX treatment (n = 6 per group). ABX: an antibiotic cocktail of 0.5 g/L Vancomycin, 1 g/L Metronidazole,1 g/L Neomycin and 1 g/L Ampicillin

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Aminoglycoside

In Vitro

Neomycin sulfate (10-500 μM; 30 min) inhibits nuclear translocation of angiogenin in HUVE cells with 60% inhibition at 100 μM and residual 14% translocation at 500 μM^[1].
Neomycin sulfate (5-50 μM; 48 h) completely abolishes angiogenin-induced proliferation of HUVE cells at 50 μM, with 49% inhibition at 5 μM and 69% inhibition at 25 μM^[1].
Neomycin sulfate (5-50 μM; 2 h) does not inhibit the ribonucleolytic activity of angiogenin against yeast tRNA at concentrations up to 50 μM^[1].
Neomycin sulfate (0.25-1.25 mM; 6 minutes after cell permeabilization) inhibits IP₃-mediated Ca²⁺ release in saponin-permeabilized RINmSF insulinoma cells in a dose-dependent manner and also inhibits the initial rate of net Ca²⁺ uptake into non-mitochondrial pools^[2].
Neomycin sulfate (1.25 mM) inhibits IP₃-mediated ⁴⁵Ca²⁺ release from saponin-permeabilized rat hepatocytes, with greater inhibition at low IP₃ concentrations^[2].
Neomycin sulfate covalently linked to glass beads avidly binds IP₃ (93% retention) and ATP (97-98% retention) and also binds PIP₂ (70% retention), but binds IP₁ and myo-inositol poorly^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	human umbilical vein endothelial (HUVE) cells
Concentration:	5, 10, 25, 50 μM
Incubation Time:	48 h
Result:	Inhibited angiogenin-induced proliferation in a dose-dependent, non-linear manner; reduced proliferation by 49% at 5 μM, by 69% at 25 μM, and completely abolished angiogenin-induced proliferation at 50 μM; did not affect basal proliferation or cell viability when used alone.

In Vivo

Neomycin sulfate (4-200 ng; applied to CAM; single dose) at 20 ng per egg completely inhibits angiogenin-induced angiogenesis in the chicken CAM model^[1].
Neomycin sulfate (1 g/L; p.o.; daily; 14 days) as part of a broad-spectrum antibiotic cocktail depletes over 90% of gut microbiota in 7.5-month-old APP/PS1 transgenic mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	SPAFAS (fertilized eggs)^[1]
Dosage:	4 ng; 20 ng; 200 ng
Administration:	applied to CAM; single dose
Result:	Decreased the percentage of angiogenin-induced positive angiogenesis from 55% to 40% with 4 ng; decreased it to 20% (equivalent to water control background) with 20 ng; decreased it to 25% with 200 ng.

Animal Model:	SPAFAS (fertilized eggs)^[1]C57BL/6J APP/PS1 (7.5 months old; 6 female, 6 male; Alzheimer's disease transgenic model)^[3]
Dosage:	1 g/L (as part of broad-spectrum antibiotic cocktail)
Administration:	p.o.; daily; 14 days (in drinking water)
Result:	Achieved >90% depletion of gut microbiota.

Molecular Weight

908.88

Formula

C₂₃H₅₂N₆O₂₅S₃

CAS No.

1405-10-3

Appearance

Solid

Color

White to light yellow

SMILES

O[C@H]1[C@H](O)[C@@H](N)[C@@H](O[C@@H]2[C@@H](N)C[C@@H](N)[C@H](O)[C@H]2O[C@@H]3O[C@H](CO)[C@@H](O[C@H]4O[C@@H](CN)[C@@H](O)[C@H](O)[C@H]4N)[C@H]3O)O[C@@H]1CN.[3H2SO4]

Structure Classification

Initial Source

Microorganisms

Micromonospora species

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 250 mg/mL (275.06 mM; Need ultrasonic)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.1003 mL	5.5013 mL	11.0026 mL
5 mM	0.2201 mL	1.1003 mL	2.2005 mL
10 mM	0.1100 mL	0.5501 mL	1.1003 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 50 mg/mL (55.01 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.86%

Select Batch:

Data Sheet (285 KB) SDS (419 KB)

COA (257 KB) HNMR (342 KB) RP-HPLC (252 KB) MS (68 KB)

Handling Instructions (2659 KB)

References

[1]. Hu GF. Neomycin inhibits angiogenin-induced angiogenesis. Proc Natl Acad Sci U S A. 1998;95(17):9791-9795. [Content Brief]

[2]. Prentki M, et al. Neomycin: a specific drug to study the inositol-phospholipid signalling system. FEBS Lett. 1986;197(1-2):285-288. [Content Brief]

[3]. Qian X, et al. Multi-omics data reveals aberrant gut microbiota-host glycerophospholipid metabolism in association with neuroinflammation in APP/PS1 mice. Gut Microbes. 2023;15(2):2282790. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	1.1003 mL	5.5013 mL	11.0026 mL	27.5064 mL
	5 mM	0.2201 mL	1.1003 mL	2.2005 mL	5.5013 mL
	10 mM	0.1100 mL	0.5501 mL	1.1003 mL	2.7506 mL
	15 mM	0.0734 mL	0.3668 mL	0.7335 mL	1.8338 mL
	20 mM	0.0550 mL	0.2751 mL	0.5501 mL	1.3753 mL
	25 mM	0.0440 mL	0.2201 mL	0.4401 mL	1.1003 mL
	30 mM	0.0367 mL	0.1834 mL	0.3668 mL	0.9169 mL
	40 mM	0.0275 mL	0.1375 mL	0.2751 mL	0.6877 mL
	50 mM	0.0220 mL	0.1100 mL	0.2201 mL	0.5501 mL
	60 mM	0.0183 mL	0.0917 mL	0.1834 mL	0.4584 mL
	80 mM	0.0138 mL	0.0688 mL	0.1375 mL	0.3438 mL
	100 mM	0.0110 mL	0.0550 mL	0.1100 mL	0.2751 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.