1. Anti-infection
  2. Bacterial
    Antibiotic
  3. Framycetin

Framycetin (Synonyms: Neomycin B; Fradiomycin B)

Cat. No.: HY-17624
Handling Instructions

Framycetin (Neomycin B), an aminoglycoside antibiotic, is a potent RNase P cleavage activity inhibitor with a Ki of 35 μM. Framycetin competes for specific divalent metal ion binding sites in RNase P RNA. Framycetin inhibits hammerhead ribozyme with a Ki of 13.5 μM. Framycetin, a 5″-azido neomycin B precursor, binds the Drosha site in miR-525 and is used for hepatic encephalopathy and enteropathogenic E. coli infections.

For research use only. We do not sell to patients.

Framycetin Chemical Structure

Framycetin Chemical Structure

CAS No. : 119-04-0

Size Price Stock Quantity
Solution
5 mg (16.27 mM * 500 μL in 0.9% NaCl) USD 60 In-stock
Estimated Time of Arrival: December 31
Solution
10 mg (16.27 mM * 1 mL in 0.9% NaCl) USD 84 In-stock
Estimated Time of Arrival: December 31

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Customer Review

Based on 1 publication(s) in Google Scholar

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Description

Framycetin (Neomycin B), an aminoglycoside antibiotic, is a potent RNase P cleavage activity inhibitor with a Ki of 35 μM. Framycetin competes for specific divalent metal ion binding sites in RNase P RNA. Framycetin inhibits hammerhead ribozyme with a Ki of 13.5 μM. Framycetin, a 5″-azido neomycin B precursor, binds the Drosha site in miR-525 and is used for hepatic encephalopathy and enteropathogenic E. coli infections[1][2].

IC50 & Target

Ki: 35 μM (RNase P cleavage activity) and 13.5 μM (hammerhead ribozyme)[1]

In Vitro

The inhibition of RNase P RNA cleavage by Framycetin (Neomycin B; Fradiomycin B) is sensitive to pH and an increase in pH suppresses the inhibition in other systems[1].
Framycetin targets the bacterial and human ribosome and affect translation. 5″-azido neomycin B and Framycetin selectively inhibit production of the mature miRNA, boosts a downstream protein, and inhibits invasion in HCC cell line[2].
Framycetin binds to a structural rather than a sequence motif of the RNA. Its primary cognate target is the decoding site of the 16S rRNA, but it also binds to the Rev-responsive element in HIV-1, group I introns, and the hammerhead ribozyme, and thus inhibits their biological function[3].
Framycetin induces misreading of the genetic code during translation and inhibits several ribozymes. The ribosomal target site is the 16 S rRNA 1400 to 1500 region[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

614.64

Formula

C23H46N6O13

CAS No.
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Solution, -20°C, 2 years

Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (162.70 mM; Need ultrasonic)

DMSO : 50 mg/mL (81.35 mM; Need ultrasonic)

In Vivo:
  • 1.

    Add each solvent one by one:  PBS

    Solubility: 120 mg/mL (195.24 mM); Clear solution; Need ultrasonic

  • 2.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (4.07 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (4.07 mM); Clear solution

  • 4.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (4.07 mM); Clear solution

*All of the co-solvents are available by MCE.
References
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Framycetin
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HY-17624
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