1. Anti-infection
  2. Bacterial Antibiotic
  3. Framycetin

Framycetin  (Synonyms: Neomycin B; Fradiomycin B)

Cat. No.: HY-17624
COA Handling Instructions

Framycetin (Neomycin B), an aminoglycoside antibiotic, is a potent RNase P cleavage activity inhibitor with a Ki of 35 μM. Framycetin competes for specific divalent metal ion binding sites in RNase P RNA. Framycetin inhibits hammerhead ribozyme with a Ki of 13.5 μM. Framycetin, a 5″-azido neomycin B precursor, binds the Drosha site in miR-525 and is used for hepatic encephalopathy and enteropathogenic E. coli infections.

For research use only. We do not sell to patients.

Framycetin Chemical Structure

Framycetin Chemical Structure

CAS No. : 119-04-0

Size Price Stock Quantity
Solvent
25 mg (16.27 mM * 2.5 mL in 0.9% NaCl) USD 50 In-stock
Solvent
50 mg (16.27 mM * 5 mL in 0.9% NaCl) USD 80 In-stock
Solvent
100 mg (16.27 mM * 10 mL in 0.9% NaCl) USD 110 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Framycetin:

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  • Biological Activity

  • Purity & Documentation

  • References

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Description

Framycetin (Neomycin B), an aminoglycoside antibiotic, is a potent RNase P cleavage activity inhibitor with a Ki of 35 μM. Framycetin competes for specific divalent metal ion binding sites in RNase P RNA. Framycetin inhibits hammerhead ribozyme with a Ki of 13.5 μM. Framycetin, a 5″-azido neomycin B precursor, binds the Drosha site in miR-525 and is used for hepatic encephalopathy and enteropathogenic E. coli infections[1][2].

IC50 & Target

Aminoglycoside

 

In Vitro

The inhibition of RNase P RNA cleavage by Framycetin (Neomycin B; Fradiomycin B) is sensitive to pH and an increase in pH suppresses the inhibition in other systems[1].
Framycetin targets the bacterial and human ribosome and affect translation. 5″-azido neomycin B and Framycetin selectively inhibit production of the mature miRNA, boosts a downstream protein, and inhibits invasion in HCC cell line[2].
Framycetin binds to a structural rather than a sequence motif of the RNA. Its primary cognate target is the decoding site of the 16S rRNA, but it also binds to the Rev-responsive element in HIV-1, group I introns, and the hammerhead ribozyme, and thus inhibits their biological function[3].
Framycetin induces misreading of the genetic code during translation and inhibits several ribozymes. The ribosomal target site is the 16 S rRNA 1400 to 1500 region[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

614.64

Formula

C23H46N6O13

CAS No.
Appearance

Liquid

Color

Colorless to light yellow

SMILES

N[C@@H](C[C@H]1N)[C@@]([C@@H]([C@H]1O)O[C@@](O[C@H](CO)[C@H]2O[C@@]([C@@H]([C@@H](O)[C@@H]3O)N)([H])O[C@H]3CN)([H])[C@@H]2O)([H])O[C@H]([C@@H]([C@@H](O)[C@@H]4O)N)O[C@@H]4CN

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Solution, -20°C, 2 years

Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (162.70 mM; Need ultrasonic)

DMSO : 50 mg/mL (81.35 mM; Need ultrasonic)

In Vivo:
  • 1.

    Add each solvent one by one:  PBS

    Solubility: 120 mg/mL (195.24 mM); Clear solution; Need ultrasonic

  • 2.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (4.07 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (4.07 mM); Clear solution

  • 4.

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (4.07 mM); Clear solution

*All of the co-solvents are available by MedChemExpress (MCE).
Purity & Documentation

References
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Framycetin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Framycetin
Cat. No.:
HY-17624
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