1. Academic Validation
  2. Lactobacillus plantarum alleviates irradiation-induced intestinal injury by activation of FXR-FGF15 signaling in intestinal epithelia

Lactobacillus plantarum alleviates irradiation-induced intestinal injury by activation of FXR-FGF15 signaling in intestinal epithelia

  • J Cell Physiol. 2022 Mar;237(3):1845-1856. doi: 10.1002/jcp.30651.
Yong-Ping Jian 1 Ge Yang 1 Li-Hong Zhang 1 Ji-Yong Liang 2 Hong-Lan Zhou 3 Yi-Shu Wang 1 Zhi-Xiang Xu 1 4
Affiliations

Affiliations

  • 1 Key Laboratory of Pathobiology, Ministry of Education, Norman Bethune College of Medicine, Jilin University, Changchun, Jilin, China.
  • 2 Department of Systems Biology, UT MD Anderson Cancer Center, Houston, Texas, USA.
  • 3 Department of Urology, The First Hospital of Jilin University, Changchun, Jilin, China.
  • 4 School of Life Sciences, Henan University, Kaifeng, Henan, China.
Abstract

Abdominal irradiation (IR) may destroy the intestinal mucosal barrier, leading to severe intestinal Infection and multiple organ dysfunction syndromes. The role of intestinal microbiota in the development of IR-induced intestinal injury remains largely unknown. Herein, we reported that abdominal IR altered the composition of the microbiota and reduced the abundance and diversity of the gut microbiome. Alterations of bacteria, in particular reduction of Lactobacillus, played a critical role in IR-induced intestinal injury. Fecal microbiota transplant (FMT) from normal mice or administration of Lactobacillus plantarum to intestinal microbiota-eliminated mice substantially reduced IR-induced intestinal damage and prevented mice from IR-induced death. We further characterized that L. plantarum activated the farnesoid X receptor (FXR) - Fibroblast Growth Factor 15 (FGF15) signaling in intestinal epithelial cells and hence promoted DNA-damage repair. Application of GW4064, an activator of FXR, to microbiota eliminated mice markedly mitigated IR-induced intestinal damage, reduced intestinal epithelial cell death and promoted the survival of IR mice. In contrast, suppression of FXR with Gly-β-MCA, a bile acid and an intestine-selective and high-affinity FXR inhibitor, abrogated L. Plantarum-mediated protection on the ileum of IR mice. Taken together, our findings not only provide new insights into the role of intestinal flora in radiation-induced intestinal injury but also shed new LIGHT on the application of probiotics for the protection of radiation-damaged individuals.

Keywords

DNA-damage repair; FXR-FGF15 axis; Lactobacillus plantarum; abdominal irradiation; intestinal epithelial injury; microbiota.

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