2. Anti-infection Metabolic Enzyme/Protease
  3. Antibiotic Phospholipase
  4. Neomycin

Neomycin, an aminoglycoside antibiotic, exerts antibacterial activity through irreversible binding of the nuclear 30S ribosomal subunit, thereby blocking bacterial protein synthesis. Neomycin is a known phospholipase C (PLC) inhibitor. Neomycin potently inhibits both the nuclear translocation of angiogenin and angiogenin-induced cell proliferation and angiogenesis. Neomycin inhibits IP3-mediated Ca2+ release, MgATP-dependent Ca2+ uptake, and electrical excitation-evoked skeletal muscle Ca2+ transients. Neomycin depletes gut microbiota in specific mouse models, causes hearing impairment, and kidney damage with prolonged exposure. Neomycin can be used for the research of cancer.

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No. CAS : 1404-04-2

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Neomycin Related Antibodies

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68 Publications Citing Use of MCE Neomycin

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    Neomycin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Apr;12(16):e2415041.  [Abstract]

    ABR thresholds in each group. The statistical differences were analyzed between Neomycin (100 mg/kg once daily for 14 days, ip) + PO group and Neomycin group.

    Neomycin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Apr;12(16):e2415041.  [Abstract]

    Effects of different levels of UA on HCs in the apical, middle, and basal turns after Neomycin (0.5 mM) treatment for 24 h.

    Neomycin purchased from MedChemExpress. Usage Cited in: Gut Microbes. 2023 Dec;15(2):2282790.  [Abstract]

    Depletion of CD8+ T cells in ABX-treated B16-F0 tumor-bearing mice dampened the antitumor effect of butyrate combined with anti-PD-1 (Figure 4b–e). Decreased serum IFN-γ and TNF-α production during the depletion of CD8+ T cells was also observed. ABX：an antibiotic cocktail of 0.5 g/L Vancomycin, 1 g/L Metronidazole, 1 g/L Neomycin and 1 g/L Ampicillin.

    Neomycin purchased from MedChemExpress. Usage Cited in: Gut Microbes. 2023 Dec;15(2):2249143.  [Abstract]

    Targeted metabolomics analysis of gut microbial metabolites (AA, PA, and BA) in colon contents (feces) and serum from mice with or without ABX treatment (n = 6 per group). ABX: an antibiotic cocktail of 0.5 g/L Vancomycin, 1 g/L Metronidazole,1 g/L Neomycin and 1 g/L Ampicillin

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    Descripciòn

    Neomycin, an aminoglycoside antibiotic, exerts antibacterial activity through irreversible binding of the nuclear 30S ribosomal subunit, thereby blocking bacterial protein synthesis. Neomycin is a known phospholipase C (PLC) inhibitor. Neomycin potently inhibits both the nuclear translocation of angiogenin and angiogenin-induced cell proliferation and angiogenesis. Neomycin inhibits IP3-mediated Ca2+ release, MgATP-dependent Ca2+ uptake, and electrical excitation-evoked skeletal muscle Ca2+ transients. Neomycin depletes gut microbiota in specific mouse models, causes hearing impairment, and kidney damage with prolonged exposure. Neomycin can be used for the research of cancer[1][2].

    In Vitro

    Neomycin (10-500 μM; 30 min) inhibits nuclear translocation of angiogenin in HUVE cells with 60% inhibition at 100 μM and residual 14% translocation at 500 μM[1].
    Neomycin (5-50 μM; 48 h) completely abolishes angiogenin-induced proliferation of HUVE cells at 50 μM, with 49% inhibition at 5 μM and 69% inhibition at 25 μM[1].
    Neomycin (5-50 μM; 2 h) does not inhibit the ribonucleolytic activity of angiogenin against yeast tRNA at concentrations up to 50 μM[1].
    Neomycin (0.25-1.25 mM; 6 minutes after cell permeabilization) inhibits IP3-mediated Ca2+ release in saponin-permeabilized RINmSF insulinoma cells in a dose-dependent manner and also inhibits the initial rate of net Ca2+ uptake into non-mitochondrial pools[2].
    Neomycin (1.25 mM) inhibits IP₃-mediated 45Ca2+ release from saponin-permeabilized rat hepatocytes, with greater inhibition at low IP₃ concentrations[2].
    Neomycin covalently linked to glass beads avidly binds IP3 (93% retention) and ATP (97-98% retention) and also binds PIP2 (70% retention), but binds IP1 and myo-inositol poorly[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Neomycin Related Antibodies

    Cell Proliferation Assay[1]

    Cell Line: human umbilical vein endothelial (HUVE) cells
    Concentration: 5, 10, 25, 50 μM
    Incubation Time: 48 h
    Result: Inhibited angiogenin-induced proliferation in a dose-dependent, non-linear manner; reduced proliferation by 49% at 5 μM, by 69% at 25 μM, and completely abolished angiogenin-induced proliferation at 50 μM; did not affect basal proliferation or cell viability when used alone.
    In Vivo

    Neomycin (4-200 ng; applied to CAM; single dose) at 20 ng per egg completely inhibits angiogenin-induced angiogenesis in the chicken CAM model[1].
    Neomycin sulfate (1 g/L; p.o.; daily; 14 days) as part of a broad-spectrum antibiotic cocktail depletes over 90% of gut microbiota in 7.5-month-old APP/PS1 transgenic mice[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: SPAFAS (fertilized eggs)[1]
    Dosage: 4 ng; 20 ng; 200 ng
    Administration: applied to CAM; single dose
    Result: Decreased the percentage of angiogenin-induced positive angiogenesis from 55% to 40% with 4 ng; decreased it to 20% (equivalent to water control background) with 20 ng; decreased it to 25% with 200 ng.
    Animal Model: C57BL/6J APP/PS1 (7.5 months old; 6 female, 6 male; Alzheimer's disease transgenic model)[3]
    Dosage: 1 g/L (as part of broad-spectrum antibiotic cocktail)
    Administration: p.o.; daily; 14 days (in drinking water)
    Result: Achieved >90% depletion of gut microbiota.
    No. CAS
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    [Neomycin]
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    Neomycin Related Classifications

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    Keywords:

    Neomycin1404-04-2AntibioticPhospholipaseRINmSF insulinoma cellsphospholipase Cangiogenesisangiogeninrat hepatocytesHUVE cellschicken CAM modelgut microbiotaAPP/PS1 transgenic miceendothelial cellsInhibitorinhibitorinhibit

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