High level 27-HC impairs trophoblast cell invasion and migration via LXR in pre-eclampsia

  • Exp Cell Res. 2024 Mar 8:113979. doi: 10.1016/j.yexcr.2024.113979.
Hai Tong  1 ,  Tingting Wang  1 ,  Ruixin Chen  2 ,  Pingsong Jin  3 ,  Youqing Jiang  3 ,  Xue Zhang  4 ,  Hongbo Qi  5
Affiliations
  • 1. Department of Obstetrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China; Chongqing Key Laboratory of Maternal and Fetal Medicine, Chongqing Medical University, Chongqing, 400016, China.
  • 2. Department of Gynecology and Obstetrics, West China Second Hospital, Sichuan University, Chengdu, 610041, China.
  • 3. Reproductive Medicine Center, The First Affiliated Hospital of Chongqing Medical University, No. 1 Youyi Road, Yuzhong District, Chongqing, 400016, China.
  • 4. Department of Obstetrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China. Electronic address: [email protected].
  • 5. Chongqing Key Laboratory of Maternal and Fetal Medicine, Chongqing Medical University, Chongqing, 400016, China; Department of Obstetrics and Gynecology, Women and Children's Hospital of Chongqing Medical University (CQMU-WCH), Chongqing, 401147, China; Joint International Research Laboratory of Reproduction and Development of Chinese Ministry of Education, Chongqing Medical University, 400016, China. Electronic address: [email protected].
PMID: 38462209 DOI: 10.1016/j.yexcr.2024.113979
Abstract

Introduction: To explore the potential impact of 27-hydroxycholesterol (27-HC) on trophoblast cell function in pre-eclampsia.

Results: The levels of 27-HC and the expression of CYP27A1 are upregulated in clinical samples of PE. Furthermore, high concentrations of 27-HC can inhibit the invasion and migration ability of trophoblast cells in vitro, and this inhibitory effect is weakened after LXR silencing. In HTR8/SVneo cells treated with 27-HC, the expression of ABCA1/ABCG1 are increased. Finally, we established a mouse model of PE using l-NAME (N-Nitro-l-Arginine Methyl Ester). We found an increase in the levels of 27-HC in the peripheral blood serum of the PE mouse model, and an upregulation of CYP27A1 and LXR expressions in the placenta of the PE mouse model.

Conclusion: 27-HC inhibits the invasion and migration ability of trophoblast cells by activating the LXR signaling pathway, which is involved in the pathogenesis of PE.

Keywords
27-Hydroxycholesterol; Invasion; LXR; Preeclampsia; Trophoblast.
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