- Fluorescent Dyes
- Fluorescence Wavelength ranges
- Emission Wavelength (Em)
- Em 591-620 nm Orange
Em 591-620 nm Orange
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Em 591-620 nm Orange (34)
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- Formula: C20H18N2O2
- Molecular Weight: 318.37
Nile red (Nile blue oxazone) is a lipophilic stain. Nile red has environment-sensitive fluorescence. Nile red is intensely fluorescent in a lipid-rich environment while it has minimal fluorescence in aqueous media. Nile red is an excellent vital stain for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytof uorometry. Nile red stains intracellular lipid droplets red. The fluorescence wavelength is 543/598 nm.
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- Formula: C21H21N3
- Molecular Weight: 315.42
Dihydroethidium, also known as DHE, is a peroxide indicator. Dihydroethidium penetrates cell membranes to form a fluorescent protein complex with blue fluoresces. After entering the cells, Dihydroethidium is mainly localized in the cell membrane, cytoplasm and nucleus, and the staining effect is the strongest in the nucleus. Dihydroethidium produces inherent blue fluorescence with a maximum excitation wavelength of 370 nm and a maximum emission wavelength of 420 nm; after dehydrogenation, Dihydroethidium combines with RNA or DNA to produce red fluorescence with a maximum excitation wavelength of 300 nm and a maximum emission wavelength of 610 nm. 535 nm can also be used as the excitation wavelength for actual observation.
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- Formula: C27H34I2N4
- Molecular Weight: 668.39
Propidium Iodide (PI) is a nuclear staining agent that stains DNA. Propidium Iodide is an analogue of ethidine bromide that emits red fluorescence upon embedding in double-stranded DNA. Propidium Iodide cannot pass through living cell membranes, but it can pass through damaged cell membranes to stain the nucleus. Propidium Iodide has a fluorescence wavelength of 493/617 nm and a wavelength of 536/635 nm after Mosaic with DNA. Propidium Iodide is commonly used in the detection of apoptosis (apoptosis) or necrosis (necrosis), and is often used in flow cytometry analysis.
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- Formula: C28H31ClN2O3
- Molecular Weight: 479.01
Rhodamine B is a staining fluorescent dye, commonly used for dyeing textiles, paper, soap, leather, and agents.
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- Formula: C72H48Cl2N6Ru
- Molecular Weight: 1169.17
Tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) dichloride ([Ru(dpp)3]2+) is an electrochemiluminescent (ECL) probe and oxygen-sensitive sensor. Tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) dichloride can be used to modify electrode surfaces for the detection of sulfates (S2O82-) and oxalates, based on electrochemical reactions that generate excited-state species, releasing photons through irreversible redox reactions. Tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) dichloride utilizes the oxygen quenching of fluorescence mechanism, where fluorescence intensity reflects the metabolic rate of living microorganisms or oxygen levels within cells/tumors, allowing for real-time detection. Tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) dichloride's main applications include microbial detection, antibacterial activity studies, and tumor microenvironment research.
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- Formula: C25H22N2O5
- Molecular Weight: 430.45
5(6)-TAMRA (5(6)-Carboxytetramethylrhodamine) is a fluorescent dye molecule widely used as a label for peptides and proteins (Ex/Em = 520/600 nm).
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- Formula: C39H50BF2N3O4S
- Molecular Weight: 705.70
The Golgi apparatus is composed of flattened vesicles superimposed on each other by unit membranes. The flattened vesicles are round with expanded and perforated edges. The Golgi fluorescent probe is a BODIPY-labeled ceramide derivative, the synthesis of which occurs in the endoplasmic reticulum and can then be transported to the Golgi via ceramide transport protein (CERT) or vesicular translocation, allowing specific labeling of the dye. BODIPY TR Ceramide (Golgi-Red Tracke) is a Golgi-specific fluorescent dye, which can visualise individual cells. Ex/Em=589 nm/616 nm.
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- Formula: C25H22N2O5
- Molecular Weight: 430.45
5(6)-TAMRA (solution) (5(6)-Carboxytetramethylrhodamine (solution)) is a fluorescent dye molecule widely used as a label for peptides and proteins (Ex/Em = 520/600 nm).
Solvent and concentration: DMSO: 10 mM
The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.
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- Formula: C44H42BClF2N6O7S2
- Molecular Weight: 915.23
ER-Tracker dye is a derivative of BODIPY series dyes coupled with Glibenclamide (HY-15206), highly selective binding to the endoplasmic reticulum, non-toxic to cells at low concentrations, this type of dye is an environmentally sensitive probe, and formaldehyde treatment can still retain part of the fluorescence, with high fluorescence life, good extinction coefficient and other characteristics. Glibenclamide is an atp-dependent K+ channel blocker (Kir6, KATP) and CFTR Cl-channel blocker that binds in the endoplasmic reticulum. ER-Tracker is not suitable for staining cells after fixation. Ex/Em=587/615 nm.
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- Formula: C31H30N2O7S2
- Molecular Weight: 606.71
Texas Red (Sulforhodamine 101) is an amphoteric rhodamine red fluorescent dye (excitation/emission: 586/605 nm). Texas Red is used extensively for investigating neuronal morphology and acts as acell type-selective fluorescent marker of astrocytes bothin vivoand in slice preparations.
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- Formula: C42H40ClN3O4
- Molecular Weight: 686.24
CellTracker Red CMTPX is a cell-permeable fluorescent dye that can be used as a cell tracer for monitoring cell movement and location (Ex/Em=586/614 nm).
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- Formula: C26H28BClF2N4O2S
- Molecular Weight: 544.85
BODIPY TR Cadaverine, a cadaverine derivative, is a red fluorescent dye. BODIPY TR Cadaverine can be used in a a highly sensitive and robust fluorescent displacement assay, which binds to native LPS strongly, specifically recognizing lipid A, and is competitively displaced by compounds displaying an affinity for lipid A.
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- Formula: C16H13As2NO3S4
- Molecular Weight: 545.38
ReAsH-EDT2 is a red fluorescent dye that marks proteins. ReAsH-EDT2 is a membrane-permeable biarsenical compound that binds covalently to tetracysteine sequences which allows the protein to be imaged. ReAsH-EDT2 can be used for protein localization and trafficking. (λex=530 nm, λem=592 nm).
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- Formula: C26H22BF2N3O4
- Molecular Weight: 489.28
BODIPY-581/591 NHS ester is a bright, red fluorescent dye (excitation: 581 nm; emission: 591 nm). BODIPY-581/591 NHS ester can be used for the labeling of amine containing biomolecules.
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- Formula: C18H23IN2
- Molecular Weight: 394.29
4-Di-2-ASP, a styryl pyridinium fluorescent dye, is a vital mitochondrial marker. 4-Di-2-ASP shows reliable and specific labelling of pulmonary NEBs (neuroepithelial bodies).
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- Formula: C34H55IN2
- Molecular Weight: 618.72
4-Di-10-ASP is a fluorescent lipophilic tracer (Excitation 485 nm; Emission 620 nm). 4-Di-10-ASP can be used to stain phospholipid membranes in a specific manner.
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- Formula: C16H19IN2
- Molecular Weight: 366.24
2-Di-1-ASP (DASPI; Compound 18a) is a mono-stryryl dye, and widely used as mitochondrial stain and groove-binding fluorescent probes for double-stranded DNA. 2-Di-1-ASP is selective for G-quadruplex (G4) and double-stranded DNA.
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- Formula: C24H18O7
- Molecular Weight: 418.40
Dihydrofluorescein diacetate is a fluorimetric probe mainly used for oxidative stress measurements, in both cell-free systems and cellular models.
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- Formula: C33H30N2O5
- Molecular Weight: 534.60
5-ROX (5-Carboxy-X-rhodamine), a rhodamine dye, exhibits strong fluorescence property in aqueous buffer with the λexit of 580 nm (ε=3.6×104 M-1 cm-1), and λemit of 604 nm (=0.94).
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- Formula: C32H31ClN2O3
- Molecular Weight: 527.05
Rhodamine 101 chloride (Rhodamine 640 chloride) is a fluorescent dye with excitation and emission maxima at 565 nM and 595 nM, respectively. Rhodamine 101 chloride binds to intracellular proteins and emits specific fluorescence. Rhodamine 101 chloride can be used in colon cancer research.
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