5-ROX (Synonyms: 5-Carboxy-X-rhodamine)

Cat. No.: HY-D0784 Purity: 99.31%: FAQs
Data Sheet SDS COA Handling Instructions

5-ROX (5-Carboxy-X-rhodamine), a rhodamine dye, exhibits strong fluorescence property in aqueous buffer with the λ_exit of 580 nm (ε=3.6×10⁴ M^-1 cm^-1), and λ_emit of 604 nm (ϕ=0.94).

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5-ROX Chemical Structure

CAS No. : 216699-35-3

Size	Price	Stock	Quantity
Solution
10 mM * 1 mL in DMSO	USD 113	In-stock	Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL ready for reconstitution	USD 113	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 96	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 156	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 480	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

5-ROX (5-Carboxy-X-rhodamine), a rhodamine dye, exhibits strong fluorescence property in aqueous buffer with the λ_exit of 580 nm (ε=3.6×10⁴ M^-1 cm^-1), and λ_emit of 604 nm (ϕ=0.94)^[1].

In Vitro

Rhodamine dyes are important because of their favorable photochemical and photophysical properties. They have long wavelength absorption maxima, high molar absorptivities, and high quantum yields^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

534.60

Appearance

Solid

Formula

C₃₃H₃₀N₂O₅

CAS No.

216699-35-3

Emission (Em)

Em 590-620 nm Orange

Excitation (Ex)

Ex 570-590 nm Yellow

SMILES

O=C(C1=CC=C(C(C2=CC3=C4N(CCC3)CCCC4=C2[O+]=C56)=C5C=C7CCCN8CCCC6=C87)C(C([O-])=O)=C1)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (233.82 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.8706 mL	9.3528 mL	18.7056 mL
5 mM	0.3741 mL	1.8706 mL	3.7411 mL
10 mM	0.1871 mL	0.9353 mL	1.8706 mL

*Please refer to the solubility information to select the appropriate solvent.

Purity & Documentation

Purity: 99.31%

Select Batch:

Data Sheet (279 KB) SDS (393 KB)

COA (259 KB) LCMS (95 KB)

Handling Instructions (2659 KB)

References

[1]. Uddin MJ, et al. Synthesis of 5- and 6-carboxy-X-rhodamines. Org Lett. 2008 Nov 6;10(21):4799-801. [Content Brief]

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Keywords:

5-ROX216699-35-35-Carboxy-X-rhodamineFluorescent DyeInhibitorinhibitorinhibit

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5-ROX (Synonyms: 5-Carboxy-X-rhodamine)

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator