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  2. BODIPY TR Cadaverine

BODIPY TR Cadaverine 

Cat. No.: HY-D1594
Handling Instructions

BODIPY TR Cadaverine, a cadaverine derivative, is a red fluorescent dye. BODIPY TR Cadaverine can be used in a a highly sensitive and robust fluorescent displacement assay, which binds to native LPS strongly, specifically recognizing lipid A, and is competitively displaced by compounds displaying an affinity for lipid A.

For research use only. We do not sell to patients.

BODIPY TR Cadaverine Chemical Structure

BODIPY TR Cadaverine Chemical Structure

CAS No. : 217190-24-4

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Description

BODIPY TR Cadaverine, a cadaverine derivative, is a red fluorescent dye. BODIPY TR Cadaverine can be used in a a highly sensitive and robust fluorescent displacement assay, which binds to native LPS strongly, specifically recognizing lipid A, and is competitively displaced by compounds displaying an affinity for lipid A[1][2].

In Vitro

BODIPY TR Cadaverine assay[1] (to quantify autophagy in cells):
Cells were incubated with 125 nM BODIPY TR Cadaverine for 10 minutes, washed four times with PBS and lysed in 10 mM Tris-Cl pH 8, 0.1 % Triton X-100. Fluorescence levels were read in a SPECTRAmax fluorimeter (Molecular Devices) (λex=588 nm, λem=616 nm). Fluorescence levels were normalized for cell number by adding 0.2 mM ethidium bromide and reading DNA fluorescence (λex=530 nm, λem=590 nm)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

544.85

Formula

C26H28BClF2N4O2S

CAS No.
SMILES

[F-][B+3]1([N-]2C(C=C(C=C3)[N]1=C3C4=CC=C(OCC(NCCCCCN)=O)C=C4)=CC=C2C5=CC=CS5)[F-].[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References
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BODIPY TR Cadaverine
Cat. No.:
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