BODIPY TR Cadaverine (solution)
Based on 1 Customer Validation
BODIPY TR Cadaverine (solution) , a cadaverine derivative, is a red fluorescent dye. BODIPY TR Cadaverine can be used in a a highly sensitive and robust fluorescent displacement assay, which binds to native LPS strongly, specifically recognizing lipid A, and is competitively displaced by compounds displaying an affinity for lipid A.
Solvent and concentration: DMSO: 2 mM
Para uso exclusivo en investigación. No vendemos a pacientes.
- No. CAS: 217190-24-4
- Fòrmula: C26H28BClF2N4O2S
- Peso molecular:544.85
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
- Pureza y Documentación
- Referencias
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Help & FAQs
Actividad biológica
Descripciòn
Solvent and concentration: DMSO: 2 mM
In Vitro
Guidelines (The following is our recommended protocol. This protocol is provided for reference only and should be modified according to your specific needs).
BODIPY TR Cadaverine assay[1] (to quantify autophagy in cells) :
Cells were incubated with 125 nM BODIPY TR Cadaverine for 10 minutes, washed four times with PBS and lysed in 10 mM Tris-Cl pH 8, 0.1 % Triton X-100. Fluorescence levels were read in a fluorimeter (Molecular Devices) (λex=588 nm, λem=616 nm). Fluorescence levels were normalized for cell number by adding 0.2 mM ethidium bromide and reading DNA fluorescence (λex=530 nm, λem=590 nm) [1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 217190-24-4
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Appearance Liquid
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Peso molecular 544.85
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Fòrmula C26H28BClF2N4O2S
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Color Pink to red
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SMILES
[F-][B+3]1([N-]2C(C=C(C=C3)[N]1=C3C4=CC=C(OCC(NCCCCCN)=O)C=C4)=CC=C2C5=CC=CS5)[F-].Cl
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureza y Documentación
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Ficha de datos (270 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Carreira RS, et al. Cyclophilin D is required for mitochondrial removal by autophagy in cardiac cells. Autophagy. 2010 May;6(4):462-72. [Content Brief]
[2]. Wood SJ, et al. Anti-endotoxin agents. 1. Development of a fluorescent probe displacement method optimized for the rapid identification of lipopolysaccharide-binding agents. Comb Chem High Throughput Screen. 2004 May;7(3):239-49. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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