Rhodamine 101 chloride
Based on 1 Customer Validation
Rhodamine 101 chloride (Rhodamine 640 chloride) is a fluorescent dye with excitation and emission maxima at 565 nM and 595 nM, respectively. Rhodamine 101 chloride binds to intracellular proteins and emits specific fluorescence. Rhodamine 101 chloride can be used in colon cancer research.
For research use only. We do not sell to patients.
- Purity: 95.0%
- CAS No.: 64339-18-0
- Formula: C32H31ClN2O3
- Molecular Weight:527.05
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Guide (The following is our recommended protocol. This protocol is for guidance only and should be modified according to your specific needs).
1 Preparation of stock solution
1) Weigh approximately 3-5 mg of Rhodamine 101 powder into 0.3 mL of DMSO.
2) Add distilled water to the above solution to adjust the final concentration of Rhodamine 101 to 1 mg/mL.
2 Preparation of working solution (for staining ethanol-fixed cells in combination with Mithramycin)
1) Dilute the stock solution with 0.15 M sodium chloride aqueous solution to adjust the final concentration of Rhodamine 101 to 1.0 μg/mL.
3 Specific Staining Steps
1) Prepare ethanol-fixed cell samples: Fix target cells (e.g., CHO cells) with 70% ethanol for at least 18 hours. Centrifuge the cell suspension to remove the ethanol fixative.
2) Resuspend the centrifuged cells in an appropriate amount of the prepared Rhodamine 101-Mithramycin dual-dye working solution.
3) Incubate the cell-dye mixture at room temperature for at least 10 minutes to ensure adequate staining.
4) After incubation, analyze the stained cells using a flow cytometer.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
595
565
Chemical Information
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CAS No. 64339-18-0
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Appearance Solid
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Molecular Weight 527.05
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Formula C32H31ClN2O3
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Color Pale purple to purple
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SMILES
O=C(C1=CC=CC=C1C(C2=CC3=C4N(CCC3)CCCC4=C2[O+]=C56)=C5C=C7CCCN8CCCC6=C87)O.[Cl-]
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Synonyms
Rhodamine 640 chloride
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
Ethanol : 14.29 mg/mL (27.11 mM; Need ultrasonic)
H2O : 2 mg/mL (3.79 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (278 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Aigner, D., et al. New fluorescent perylene bisimide indicators--a platform for broadband pH optodes. Anal. Bioanal. Chem. 400(8), 2475-2485 (2011). [Content Brief]
[2]. Crissman, H.A., et al. Rapid, one step staining procedures for analysis of cellular DNA and protein by single and dual laser flow cytometry. Cytometry 3(2), 84-90 (1982). [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / Ethanol | 1 mM | 1.8974 mL | 9.4868 mL | 18.9735 mL | 47.4338 mL |
| Ethanol | 5 mM | 0.3795 mL | 1.8974 mL | 3.7947 mL | 9.4868 mL |
| 10 mM | 0.1897 mL | 0.9487 mL | 1.8974 mL | 4.7434 mL | |
| 15 mM | 0.1265 mL | 0.6325 mL | 1.2649 mL | 3.1623 mL | |
| 20 mM | 0.0949 mL | 0.4743 mL | 0.9487 mL | 2.3717 mL | |
| 25 mM | 0.0759 mL | 0.3795 mL | 0.7589 mL | 1.8974 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Rhodamine 101 chloride Related Classifications
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