Rhodamine 101 (inner salt)

Guide (The following is our recommended protocol. This protocol is for guidance only and should be modified according to your specific needs).
1 Preparation of stock solution
1) Weigh approximately 3-5 mg of Rhodamine 101 powder into 0.3 mL of DMSO.
2) Add distilled water to the above solution to adjust the final concentration of Rhodamine 101 to 1 mg/mL.
2 Preparation of working solution (for staining ethanol-fixed cells in combination with Mithramycin)
1) Dilute the stock solution with 0.15 M sodium inner salt aqueous solution to adjust the final concentration of Rhodamine 101 to 1.0 μg/mL.
3 Specific Staining Steps
1) Prepare ethanol-fixed cell samples: Fix target cells (e.g., CHO cells) with 70% ethanol for at least 18 hours. Centrifuge the cell suspension to remove the ethanol fixative.
2) Resuspend the centrifuged cells in an appropriate amount of the prepared Rhodamine 101-Mithramycin dual-dye working solution.
3) Incubate the cell-dye mixture at room temperature for at least 10 minutes to ensure adequate staining.
4) After incubation, analyze the stained cells using a flow cytometer.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.