CellTracker Red CMTPX 

CellTracker Red CMTPX is a cell-permeable fluorescent dye that can be used as a cell tracer for monitoring cell movement and location (Ex/Em=586/614 nm)^[1].

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Fixation and staining of self-assembled spheroids^[1]:
1. Resuspended cells are stained with CellTracker Red CMTPX and allowed to self-assemble into spheroids before aggregation.
2. Cell aggregates are fixed in 4% paraformaldehyde/PBS for 30 min with gentle inversion, then washing cells in PBS for 3 times and stained with 100 ng/mL Hoechst dye overnight prior to imaging.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

686.24

C₄₂H₄₀ClN₃O₄

Oil

Blue to dark blue

614

586

CC1=CC(C)(C)N(C)C2=CC3=C(C(C4=C(C([O-])=O)C=CC(NC(C5=CC=C(CCl)C=C5)=O)=C4)=C6C=C7C(C)=CC(C)(C)[N+](C)=C7C=C6O3)C=C12.CC8=CC(C)(C)N(C)C9=CC%10=C(C(C%11=C(C([O-])=O)C=C(NC(C%12=CC=C(CCl)C=C%12)=O)C=C%11)=C%13C=C%14C(C)=CC(C)(C)[N+](C)=C%14C=C%13O%10)C=C89

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

• [1]. Van Pel DM, et al. Modelling glioma invasion using 3D bioprinting and scaffold-free 3D culture. J Cell Commun Signal. 2018 Dec;12(4):723-730.  [Content Brief]