Rhodamine B
Based on 24 publication(s) in Google Scholar
Rhodamine B is a staining fluorescent dye, commonly used for dyeing textiles, paper, soap, leather, and agents.
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
- Pureté: 98.94%
- CAS No.: 81-88-9
- Formule: C28H31ClN2O3
- Masse moléculaire:479.01
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Stockage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Rhodamine B
More- Adv Mater. 2026 Apr 24:e73114.
- ACS Nano. 2024 Mar 26;18(12):8777-8797. [Abstract]
- Nat Commun. 2026 Apr 24;17(1):5658.
- Chem Eng J. 2020, 127870.
- Biomaterials. 2026 Sep:332:124156. [Abstract]
- Biomaterials. 2025 May 5:322:123391. [Abstract]
- Biomaterials. 2025 Jan:312:122742. [Abstract]
- J Nanobiotechnology. 2026 May 3;24(1):586.
- J Nanobiotechnology. 2025 Oct 27;23(1):690. [Abstract]
- J Nanobiotechnology. 2025 Jun 13;23(1):443. [Abstract]
- J Control Release. 2026 Jul 10:395:114937.
- Mater Today Bio. 2026 Mar 31:38:103086. [Abstract]
- Adv Healthc Mater. 2025 Jul 16:e2501440. [Abstract]
- Int J Biol Macromol. 2024 May 17;270(Pt 1):132432. [Abstract]
- EMBO J. 2020 Sep 15;39(18):e104365. [Abstract]
- Int J Nanomedicine. 2024 Jun 24:19:6337-6358. [Abstract]
- ACS Appl Bio Mater. 2026 Feb 2;9(3):1352-1364. [Abstract]
- Nanomedicine. 2026 Mar;21(6):789-801. [Abstract]
- Biomed J. 2024 Feb;47(1):100592. [Abstract]
- ACS Omega. 2022 Dec 23;8(1):1710-1722. [Abstract]
- ACS Synth Biol. 2025 Sep 19;14(9):3767-3783. [Abstract]
- PLoS Genet. 2026 Apr 30;22(4):e1012119.
- Biomed Res Int. 2022 Nov 12:2022:6441179. [Abstract]
- Biochim Biophys Acta Gen Subj. 2025 Aug 13;1869(11):130851. [Abstract]
Customer Validation & Images
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Others
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Cell Imaging/Staining
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Cell Imaging/Staining
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Cell Imaging/Staining
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In Vivo Imaging
Activité biologique
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| 518A2 | EC50 |
>30 μM
Compound: Rhodamine B
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Cytotoxicity against human 518A2 cells after 96 hrs by SRB assay
Cytotoxicity against human 518A2 cells after 96 hrs by SRB assay
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[PMID: 28033541] |
| 8505C | EC50 |
>30 μM
Compound: Rhodamine B
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Cytotoxicity against human 8505C cells after 96 hrs by SRB assay
Cytotoxicity against human 8505C cells after 96 hrs by SRB assay
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[PMID: 28033541] |
| A2780 | EC50 |
>30 μM
Compound: Rhodamine B
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Cytotoxicity against human A2780 cells after 96 hrs by SRB assay
Cytotoxicity against human A2780 cells after 96 hrs by SRB assay
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[PMID: 28033541] |
| A2780 | EC50 |
>30 μM
Compound: Rhodamine B
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Cytotoxicity against human A2780 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay
Cytotoxicity against human A2780 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay
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[PMID: 33049606] |
| A2780 | EC50 |
>30 μM
Compound: 5
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Cytotoxicity against human A2780 cells incubated for 72 hrs by SRB assay
Cytotoxicity against human A2780 cells incubated for 72 hrs by SRB assay
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[PMID: 36780832] |
| A-375 | EC50 |
>30 μM
Compound: Rhodamine B
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Cytotoxicity against human A-375 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay
Cytotoxicity against human A-375 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay
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[PMID: 33049606] |
| A-375 | EC50 |
>30 μM
Compound: 5
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Cytotoxicity against human A-375 cells incubated for 72 hrs by SRB assay
Cytotoxicity against human A-375 cells incubated for 72 hrs by SRB assay
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[PMID: 36780832] |
| A549 | EC50 |
>30 μM
Compound: Rhodamine B
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Cytotoxicity against human A549 cells after 96 hrs by SRB assay
Cytotoxicity against human A549 cells after 96 hrs by SRB assay
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[PMID: 28033541] |
| FaDu | EC50 |
>30 μM
Compound: Rhodamine B
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Cytotoxicity against human FaDu cells assessed as reduction in cell viability measured after 72 hrs by SRB assay
Cytotoxicity against human FaDu cells assessed as reduction in cell viability measured after 72 hrs by SRB assay
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[PMID: 33049606] |
| HEK293 | EC50 |
>30 μM
Compound: Rhodamine B
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Cytotoxicity against human HEK293 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay
Cytotoxicity against human HEK293 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay
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[PMID: 33049606] |
| HT-29 | EC50 |
>30 μM
Compound: Rhodamine B
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Cytotoxicity against human HT-29 cells after 96 hrs by SRB assay
Cytotoxicity against human HT-29 cells after 96 hrs by SRB assay
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[PMID: 28033541] |
| HT-29 | EC50 |
>30 μM
Compound: Rhodamine B
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Cytotoxicity against human HT-29 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay
Cytotoxicity against human HT-29 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay
|
[PMID: 33049606] |
| HT-29 | EC50 |
>30 μM
Compound: 5
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Cytotoxicity against human HT-29 cells incubated for 72 hrs by SRB assay
Cytotoxicity against human HT-29 cells incubated for 72 hrs by SRB assay
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[PMID: 36780832] |
| MCF7 | EC50 |
>30 μM
Compound: Rhodamine B
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Cytotoxicity against human MCF7 cells after 96 hrs by SRB assay
Cytotoxicity against human MCF7 cells after 96 hrs by SRB assay
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[PMID: 28033541] |
| MCF7 | EC50 |
>30 μM
Compound: Rhodamine B
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Cytotoxicity against human MCF7 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay
Cytotoxicity against human MCF7 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay
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[PMID: 33049606] |
| MCF7 | EC50 |
>30 μM
Compound: 5
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Cytotoxicity against human MCF7 cells incubated for 72 hrs by SRB assay
Cytotoxicity against human MCF7 cells incubated for 72 hrs by SRB assay
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[PMID: 36780832] |
| NIH3T3 | EC50 |
>30 μM
Compound: Rhodamine B
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Cytotoxicity against mouse NIH/3T3 cells after 96 hrs by SRB assay
Cytotoxicity against mouse NIH/3T3 cells after 96 hrs by SRB assay
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[PMID: 28033541] |
| NIH3T3 | EC50 |
>30 μM
Compound: Rhodamine B
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Cytotoxicity against mouse NIH3T3 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay
Cytotoxicity against mouse NIH3T3 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay
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[PMID: 33049606] |
| NIH3T3 | EC50 |
>30 μM
Compound: 5
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Cytotoxicity against mouse NIH/3T3 cells incubated for 72 hrs by SRB assay
Cytotoxicity against mouse NIH/3T3 cells incubated for 72 hrs by SRB assay
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[PMID: 36780832] |
Rhodamine B induces a concentration-dependent reduction of root meristem cells of A. cepa. mitotic activity. Rhodamine B induces various nuclear aberrations in A. cepa. root cells. In the 100 and 200 ppm rhodamine B groups, the frequencies of NBUDs and BN surpass those of the positive control (MMS) group. Rhodamine B-induced changes of H2O2 (a) and MDA (b) level increase in a concentration-dependent manner in A. cepa. roots[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Chemical Information
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CAS No. 81-88-9
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Appearance Solid
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Masse moléculaire 479.01
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Formule C28H31ClN2O3
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Color Dark purple to black
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SMILES
CCN(C1=CC2=[O+]C3=C(C=CC(N(CC)CC)=C3)C(C4=CC=CC=C4C(O)=O)=C2C=C1)CC.[Cl-]
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Synonyms
Basic Violet 10; Brilliant Pink B; Rhodamine O; Tetraethylrhodamine
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light)
Publications (24)
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Journal Impact Factor
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Most Recent
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ACS Nano
Osteoinductive Dental Pulp Stem Cell-Derived Extracellular Vesicle-Loaded Multifunctional Hydrogel for Bone Regeneration. [Abstract]2024 Mar 26;18(12):8777-8797. PMID: 38488479
Rhodamine B purchased from MedChemExpress. Usage Cited in: ACS Nano. 2024 Mar 26;18(12):8777-8797. [Abstract]
Fluorescence images showing the retention of Rhodamine B-labeled Ost-EVs after intramuscular injection. Before experiments, Ost-EVs were incubated with 10 μM Rhodamine B for 2 h and ultrafiltrated (10 kDa) at 14 000 × g for 15 min. Then, the Ost-EV-laden hydrogels were intramuscularly injected into the muscles of the rat’s hind limb. Ost-EVs in equivalent PBS were used as controls.
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Biomaterials
Reversing neuroinflammation in ischemic stroke through efferocytotic phenotype reprogramming with polymeric nanoparticles. [Abstract]2026 Sep:332:124156. PMID: 41895020 -
Biomaterials
Radiation-triggerable bioreactors enable bioenergetic reprograming of cancer stem cell plasticity via targeted arginine metabolism disruption for augmented radio-immunotherapy. [Abstract]2025 May 5:322:123391. PMID: 40344881
Rhodamine B purchased from MedChemExpress. Usage Cited in: Biomaterials. 2025 May 5:322:123391. [Abstract]
Design and construction of RTBs for remodeling CSC plasticity. Fluorescence emission spectra of FITC-RTB-RhB at 488 nm. (FITC: HY-66019; RhB, Rhodamine B, HY-Y0016)
Rhodamine B purchased from MedChemExpress. Usage Cited in: Biomaterials. 2025 May 5:322:123391. [Abstract]
CLSM images showing the time-dependent changes in the intracellular localization of RhB (Rhodamine B)-labeled pDNAiNOS after incubating Hep1-6-derived HCSCs with RTB (pDNAiNOS-RhB), lysosomes were labeled green with Lyso-TrackerGreen, and cell nuclei were labeled blue with Hoechst.
Rhodamine B purchased from MedChemExpress. Usage Cited in: Biomaterials. 2025 May 5:322:123391. [Abstract]
CLSM analysis on the subcellular co-localization of RhB (Rhodamine B)-labeled pDNAiNOS and Nfr2 after incubating Hep1-6-derived HCSCs with pDNAiNOS-RhB-based RTBs with or without LDR.
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Biomaterials
Single-dose of integrated bilayer microneedles for enhanced hypertrophic scar therapy with rapid anti-inflammatory and sustained inhibition of myofibroblasts. [Abstract]2025 Jan:312:122742. PMID: 39106821 -
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J Nanobiotechnology
Dual-function MgSrCeAl-LDH nanosheets enable both ROS clearance and enhanced angiogenesis for myocardial ischemia/reperfusion injury therapy. [Abstract]2025 Oct 27;23(1):690. PMID: 41139778 -
J Nanobiotechnology
Overcoming acquired immunotherapy resistance in non-small cell lung cancer using ginsenoside Rb1-loaded, peptide-enhanced exosome delivery systems. [Abstract]2025 Jun 13;23(1):443. PMID: 40514658
Rhodamine B purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2025 Jun 13;23(1):443. [Abstract]
Colocalization of PKH-67-exo and Rhd-TMTP1 observed under a confocal laser scanning microscope (white arrows indicate colocalized PKH-67-exo and Rhd-TMTP1, scale bar = 15 μm). Rhd (Rhodamine), HY-Y0016; PKH-67, HY-D1421.
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Mater Today Bio
Mannosylated graphene oxide nanotherapeutics co-delivering docetaxel and a STING agonist reprogram myeloid cells and potentiate antitumor immunity. [Abstract]2026 Mar 31:38:103086. PMID: 42006712 -
Adv Healthc Mater
Triple-Tailored Analgesic Hydrogel System Targeting ADAM17 in Orofacial Inflammatory Pain. [Abstract]2025 Jul 16:e2501440. PMID: 40671384 -
Int J Biol Macromol
Advancing vaccine development: Evaluation of a mannose-modified lipid nanoparticle-based candidate for African swine fever p30 mRNA vaccine eliciting robust immune response in mice. [Abstract]2024 May 17;270(Pt 1):132432. PMID: 38761609 -
EMBO J
2020 Sep 15;39(18):e104365. PMID: 32696520 -
Int J Nanomedicine
pH-Responsive Mesoporous Silica Nanoparticles Loaded with Naringin for Targeted Osteoclast Inhibition and Bone Regeneration. [Abstract]2024 Jun 24:19:6337-6358. PMID: 38946884 -
ACS Appl Bio Mater
PEGylated Graphene Oxide as a Nanocarrier Assists Alpha Lipoic Acid Mitigating Hypoxia Induced Mitochondrial Dysfunction. [Abstract]2026 Feb 2;9(3):1352-1364. PMID: 41535114 -
Nanomedicine
Precise atorvastatin delivery by cardiac homing peptide functionalized nanoliposomes for myocardial damage repair after myocardial infarction. [Abstract]2026 Mar;21(6):789-801. PMID: 41660754 -
Biomed J
2024 Feb;47(1):100592. PMID: 37004870 -
ACS Omega
2022 Dec 23;8(1):1710-1722. PMID: 36643556 -
ACS Synth Biol
Signal Peptide-Guided Delivery of a Mucin-Like Collagen Analogue for Periplasmic Barrier Reinforcement: A Platform for Enhancing Microbial Survival. [Abstract]2025 Sep 19;14(9):3767-3783. PMID: 40924575 -
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Biomed Res Int
Multifunctional Drug-Loaded Phase-Change Nanoparticles Inhibit the Epithelial-Mesenchymal Transition of Hepatocellular Carcinoma by Affecting the Activity of Activated Hepatic Stellate Cells. [Abstract]2022 Nov 12:2022:6441179. PMID: 36411770 -
Biochim Biophys Acta Gen Subj
Unraveling the power of TOMM40: Driving PHB1-mediated mtDNA release and mitophagy to fuel breast cancer progression. [Abstract]2025 Aug 13;1869(11):130851. PMID: 40816542
Solvant et solubilité
DMSO : 125 mg/mL (260.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 100 mg/mL (208.76 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureté et documentation
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Fiche technique (277 KB)
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SDS (787 KB)
- English - EN (787 KB)
- Français - FR (787 KB)
- Deutsch - DE (787 KB)
- Norwegian - NO (787 KB)
- Español - ES (787 KB)
- Swedish - SV (787 KB)
- Italian - IT (787 KB)
- Korean - KR (787 KB)
- Portuguese - PT (787 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 2.0876 mL | 10.4382 mL | 20.8764 mL | 52.1910 mL |
| 5 mM | 0.4175 mL | 2.0876 mL | 4.1753 mL | 10.4382 mL | |
| 10 mM | 0.2088 mL | 1.0438 mL | 2.0876 mL | 5.2191 mL | |
| 15 mM | 0.1392 mL | 0.6959 mL | 1.3918 mL | 3.4794 mL | |
| 20 mM | 0.1044 mL | 0.5219 mL | 1.0438 mL | 2.6095 mL | |
| 25 mM | 0.0835 mL | 0.4175 mL | 0.8351 mL | 2.0876 mL | |
| 30 mM | 0.0696 mL | 0.3479 mL | 0.6959 mL | 1.7397 mL | |
| 40 mM | 0.0522 mL | 0.2610 mL | 0.5219 mL | 1.3048 mL | |
| 50 mM | 0.0418 mL | 0.2088 mL | 0.4175 mL | 1.0438 mL | |
| 60 mM | 0.0348 mL | 0.1740 mL | 0.3479 mL | 0.8698 mL | |
| 80 mM | 0.0261 mL | 0.1305 mL | 0.2610 mL | 0.6524 mL | |
| 100 mM | 0.0209 mL | 0.1044 mL | 0.2088 mL | 0.5219 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Rhodamine B Related Classifications
HY-Y0016 Related Classifications
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