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Cat. No.: HY-DY1077

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Rhodamine B (solution)

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Rhodamine B (solution) is a staining fluorescent dye, commonly used for dyeing textiles, paper, soap, leather, and agents.
Solvent and concentration: DMSO: 10 mM

For research use only. We do not sell to patients.

CAS No.: 81-88-9
Formula: C₂₈H₃₁ClN₂O₃
Molecular Weight:479.02

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Rhodamine B (solution) Chemical Structure

CAS No.: 81-88-9

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Rhodamine B (solution) is a staining fluorescent dye, commonly used for dyeing textiles, paper, soap, leather, and agents.
Solvent and concentration: DMSO: 10 mM

Cellular Effect

Cell Line	Type	Value	Description	References
518A2	EC₅₀	>30 μM Compound: Rhodamine B	Cytotoxicity against human 518A2 cells after 96 hrs by SRB assay Cytotoxicity against human 518A2 cells after 96 hrs by SRB assay	[PMID: 28033541]
8505C	EC₅₀	>30 μM Compound: Rhodamine B	Cytotoxicity against human 8505C cells after 96 hrs by SRB assay Cytotoxicity against human 8505C cells after 96 hrs by SRB assay	[PMID: 28033541]
A2780	EC₅₀	>30 μM Compound: 5	Cytotoxicity against human A2780 cells incubated for 72 hrs by SRB assay Cytotoxicity against human A2780 cells incubated for 72 hrs by SRB assay	[PMID: 36780832]
A2780	EC₅₀	>30 μM Compound: Rhodamine B	Cytotoxicity against human A2780 cells after 96 hrs by SRB assay Cytotoxicity against human A2780 cells after 96 hrs by SRB assay	[PMID: 28033541]
A2780	EC₅₀	>30 μM Compound: Rhodamine B	Cytotoxicity against human A2780 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay Cytotoxicity against human A2780 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay	[PMID: 33049606]
A-375	EC₅₀	>30 μM Compound: 5	Cytotoxicity against human A-375 cells incubated for 72 hrs by SRB assay Cytotoxicity against human A-375 cells incubated for 72 hrs by SRB assay	[PMID: 36780832]
A-375	EC₅₀	>30 μM Compound: Rhodamine B	Cytotoxicity against human A-375 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay Cytotoxicity against human A-375 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay	[PMID: 33049606]
A549	EC₅₀	>30 μM Compound: Rhodamine B	Cytotoxicity against human A549 cells after 96 hrs by SRB assay Cytotoxicity against human A549 cells after 96 hrs by SRB assay	[PMID: 28033541]
FaDu	EC₅₀	>30 μM Compound: Rhodamine B	Cytotoxicity against human FaDu cells assessed as reduction in cell viability measured after 72 hrs by SRB assay Cytotoxicity against human FaDu cells assessed as reduction in cell viability measured after 72 hrs by SRB assay	[PMID: 33049606]
HEK293	EC₅₀	>30 μM Compound: Rhodamine B	Cytotoxicity against human HEK293 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay Cytotoxicity against human HEK293 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay	[PMID: 33049606]
HT-29	EC₅₀	>30 μM Compound: 5	Cytotoxicity against human HT-29 cells incubated for 72 hrs by SRB assay Cytotoxicity against human HT-29 cells incubated for 72 hrs by SRB assay	[PMID: 36780832]
HT-29	EC₅₀	>30 μM Compound: Rhodamine B	Cytotoxicity against human HT-29 cells after 96 hrs by SRB assay Cytotoxicity against human HT-29 cells after 96 hrs by SRB assay	[PMID: 28033541]
HT-29	EC₅₀	>30 μM Compound: Rhodamine B	Cytotoxicity against human HT-29 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay Cytotoxicity against human HT-29 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay	[PMID: 33049606]
MCF7	EC₅₀	>30 μM Compound: 5	Cytotoxicity against human MCF7 cells incubated for 72 hrs by SRB assay Cytotoxicity against human MCF7 cells incubated for 72 hrs by SRB assay	[PMID: 36780832]
MCF7	EC₅₀	>30 μM Compound: Rhodamine B	Cytotoxicity against human MCF7 cells after 96 hrs by SRB assay Cytotoxicity against human MCF7 cells after 96 hrs by SRB assay	[PMID: 28033541]
MCF7	EC₅₀	>30 μM Compound: Rhodamine B	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay	[PMID: 33049606]
NIH3T3	EC₅₀	>30 μM Compound: 5	Cytotoxicity against mouse NIH/3T3 cells incubated for 72 hrs by SRB assay Cytotoxicity against mouse NIH/3T3 cells incubated for 72 hrs by SRB assay	[PMID: 36780832]
NIH3T3	EC₅₀	>30 μM Compound: Rhodamine B	Cytotoxicity against mouse NIH/3T3 cells after 96 hrs by SRB assay Cytotoxicity against mouse NIH/3T3 cells after 96 hrs by SRB assay	[PMID: 28033541]
NIH3T3	EC₅₀	>30 μM Compound: Rhodamine B	Cytotoxicity against mouse NIH3T3 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay Cytotoxicity against mouse NIH3T3 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay	[PMID: 33049606]

In Vitro

Rhodamine B induces a concentration-dependent reduction of root meristem cells of A. cepa. mitotic activity. Rhodamine B induces various nuclear aberrations in A. cepa. root cells. In the 100 and 200 ppm rhodamine B groups, the frequencies of NBUDs and BN surpass those of the positive control (MMS) group. Rhodamine B-induced changes of H₂O₂ (a) and MDA (b) level increase in a concentration-dependent manner in A. cepa. roots^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 81-88-9
Appearance Liquid
Molecular Weight 479.02
Formula C₂₈H₃₁ClN₂O₃
Color Orange to red
SMILES

CCN(C1=CC2=[O+]C3=C(C=CC(N(CC)CC)=C3)C(C4=CC=CC=C4C(O)=O)=C2C=C1)CC.[Cl-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (271 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Tan D, et al. Rhodamine B induces long nucleoplasmic bridges and other nuclear anomalies in Allium cepa root tip cells. Environ Sci Pollut Res Int. 2014 Mar;21(5):3363-70. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

100 mg

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