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Texas Red  (Synonyms: Sulforhodamine 101; SR101)

Cat. No.: HY-101878 Purity: 99.72%
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Texas Red (Sulforhodamine 101) is an amphoteric rhodamine red fluorescent dye (excitation/emission: 586/605 nm). Texas Red is used extensively for investigating neuronal morphology and acts as acell type-selective fluorescent marker of astrocytes bothin vivoand in slice preparations.

For research use only. We do not sell to patients.

CAS No. : 60311-02-6

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Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of Texas Red:

Texas Red Related Antibodies

Top Publications Citing Use of Products

    Texas Red purchased from MedChemExpress. Usage Cited in: Neuron. 2022 Sep 21;110(18):2984-2999.e8.  [Abstract]

    The astrocyte-specific dye Texas Red (Sulforhodamine 101; SR101) (1 μM; 15 min; 37 ℃) was used for two-photon Ca2+ imaging of hippocampal slices.

    Texas Red purchased from MedChemExpress. Usage Cited in: Neuropharmacology. 2022 Oct 1:217:109191.  [Abstract]

    Example image of a patch-clamp recorded astrocyte labeled with Texas Red (Sulforhodamine 101; SR101) (5 μM; 35 min; 34 ℃) in the alveus of the hippocampal CA1 region.

    Texas Red purchased from MedChemExpress. Usage Cited in: Neuropharmacology. 2022 Oct 1:217:109191.  [Abstract]

    Example images of astrocytes in hippocampal slices labeled with Texas Red (Sulforhodamine 101; SR101) (5 μM; 35 min; 34 ℃) and filled with Fluo-4, a calcium indicator. Arrows indicate astrocytes co-labeled with Texas Red (Sulforhodamine 101; SR101) and Fluo-4.

    Texas Red purchased from MedChemExpress. Usage Cited in: J Biomed Res. 2022 Aug 10;36(5):358-367.  [Abstract]

    The trajectory of astrocytic Ca2+ signal-weighted centroid from C57BL/6J mice. The grey cellular surface was reconstructed from Texas Red (Sulforhodamine 101; SR101) (5 mM; i.v.; 1 h) labeling. The overlapped red trace was the projection of signal centroid.

    Texas Red purchased from MedChemExpress. Usage Cited in: Glia. 2021 Feb;69(2):281-295.  [Abstract]

    Representative fluorescence images showed Na+-insensitive fluorescent dye Texas Red (Sulforhodamine 101; SR101) (1 μM; 30 min; 37 ℃) trapped inside astroglial cytoplasm and counterstained with Hoechst 33342.

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Texas Red (Sulforhodamine 101) is an amphoteric rhodamine red fluorescent dye (excitation/emission: 586/605 nm). Texas Red is used extensively for investigating neuronal morphology and acts as acell type-selective fluorescent marker of astrocytes bothin vivoand in slice preparations[1].

    In Vitro

    Guidelines (The following is our recommended protocol. This protocol is provided for reference only and should be modified according to your specific needs).
    Texas Red (Sulforhodamine 101) can be used as an astrocyte marker for hippocampal slices staining with red fluorescence[3].
    For cell staining.
    1. Stain hippocampal slices with Texas Red (5 μM; 35 min; 34°C) in carbogenated Artificial CerebroSpinal Fluid (ACSF).
    2. Return hippocampal slices to dye-free ACSF for at least 30 min before imaging.
    3. Use a confocal laser scanning microscopy (Zeiss LSM 880) for image.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Texas Red Related Antibodies
    In Vivo

    In vivo, epileptic activity can be induced by intra-hippocampal injection of small volumes of 10 μM Texas Red or topical application of 100 μM[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    606.71

    Formula

    C31H30N2O7S2
    CAS No.
    Appearance

    Solid

    Color

    Green to dark green

    Emission (Em)

    605
    Excitation (Ex)

    586
    SMILES

    O=S(C1=CC=C(C(C2=CC3=C4N(CCC3)CCCC4=C2[O+]=C56)=C5C=C7CCCN8CCCC6=C87)C(S(=O)([O-])=O)=C1)(O)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, protect from light

    *The compound is unstable in solutions, freshly prepared is recommended.

    Solvent & Solubility
    In Vitro: 

    DMSO : 31.82 mg/mL (52.45 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 8.33 mg/mL (13.73 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.6482 mL 8.2412 mL 16.4823 mL
    5 mM 0.3296 mL 1.6482 mL 3.2965 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.72%

    SDS (393 KB)

    COA (261 KB) LCMS (93 KB)

    Handling Instructions (2659 KB)
    Dyeing Example
    References
    Cell Assay
    [1]

    Acute brain slices are usually incubated in carbonated extracellular solution containing 0.5 to1 μM Texas Red for 20 to 30 min and 34 to 37°C. Following this, excess dye is removed over a period of 10 to 30 min using different protocols that were described earlier[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [1]

    For in vivo imaging, Texas Red (Sulforhodamine 101) is applied topically at concentrations of 250 nM to 300 μM or by bolus injection. Additionally, Texas Red injection over the tail vein (10 mg/mL) has been reported to be successful[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 1.6482 mL 8.2412 mL 16.4823 mL 41.2058 mL
    5 mM 0.3296 mL 1.6482 mL 3.2965 mL 8.2412 mL
    10 mM 0.1648 mL 0.8241 mL 1.6482 mL 4.1206 mL
    DMSO 15 mM 0.1099 mL 0.5494 mL 1.0988 mL 2.7471 mL
    20 mM 0.0824 mL 0.4121 mL 0.8241 mL 2.0603 mL
    25 mM 0.0659 mL 0.3296 mL 0.6593 mL 1.6482 mL
    30 mM 0.0549 mL 0.2747 mL 0.5494 mL 1.3735 mL
    40 mM 0.0412 mL 0.2060 mL 0.4121 mL 1.0301 mL
    50 mM 0.0330 mL 0.1648 mL 0.3296 mL 0.8241 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Texas Red Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Texas Red60311-02-6Sulforhodamine 101 SR101Sulforhodamine101Sulforhodamine-101SR101SR 101SR-101Fluorescent DyeastrocyterhodamineneuronalmorphologyliposomepcrInhibitorinhibitorinhibit

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