1. Dye Reagents
  2. Sulforhodamine 101

Sulforhodamine 101 (Synonyms: SR101)

Cat. No.: HY-101878 Purity: 99.21%
Handling Instructions

Sulforhodamine 101 (SR101) is an amphoteric rhodamine red fluorescent dye (excitation/emission: 586/605 nm). Sulforhodamine 101 is used extensively for investigating neuronal morphology and acts as a cell type-selective fluorescent marker of astrocytes both in vivo and in slice preparations.

For research use only. We do not sell to patients.

Sulforhodamine 101 Chemical Structure

Sulforhodamine 101 Chemical Structure

CAS No. : 60311-02-6

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25 mg USD 60 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

Sulforhodamine 101 (SR101) is an amphoteric rhodamine red fluorescent dye (excitation/emission: 586/605 nm). Sulforhodamine 101 is used extensively for investigating neuronal morphology and acts as a cell type-selective fluorescent marker of astrocytes both in vivo and in slice preparations[1].

In Vitro

Sulforhodamine 101 (SR101) does not label astrocytes in brainstem slices as strong and specific as in the hippocampus or cortex. To minimize excitatory side effects, the concentration of Sulforhodamine 101 has to be kept as low as possible or the labeling procedure can be performed after the actual experiment[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

In vivo, epileptic activity can be induced by intra-hippocampal injection of small volumes of 10 μM Sulforhodamine 101 or topical application of 100 μM[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

606.71

Formula

C₃₁H₃₀N₂O₇S₂

CAS No.
SMILES

O=S(C1=CC=C(C(C2=CC3=C4N(CCC3)CCCC4=C2[O+]=C56)=C5C=C7CCCN8CCCC6=C87)C(S(=O)([O-])=O)=C1)(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility
In Vitro: 

DMSO : ≥ 75 mg/mL (123.62 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6482 mL 8.2412 mL 16.4823 mL
5 mM 0.3296 mL 1.6482 mL 3.2965 mL
10 mM 0.1648 mL 0.8241 mL 1.6482 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (3.43 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (3.43 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Cell Assay
[1]

Acute brain slices are usually incubated in carbonated extracellular solution containing 0.5 to1 μM Sulforhodamine 101 for 20 to 30 min and 34 to 37°C. Following this, excess dye is removed over a period of 10 to 30 min using different protocols that were described earlier[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[1]

For in vivo imaging, Sulforhodamine 101 (SR101) is applied topically at concentrations of 250 nM to 300 μM or by bolus injection. Additionally, Sulforhodamine 101 injection over the tail vein (10 mg/mL) has been reported to be successful[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: 99.21%

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Keywords:

Sulforhodamine 101SR101Sulforhodamine101Sulforhodamine-101SR 101SR-101OthersastrocyterhodamineneuronalmorphologyliposomepcrInhibitorinhibitorinhibit

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Product Name:
Sulforhodamine 101
Cat. No.:
HY-101878
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