Texas Red (solution)
Texas Red (Sulforhodamine 101) (solution) is an amphoteric rhodamine red fluorescent dye (excitation/emission: 586/605 nm). Texas Red is used extensively for investigating neuronal morphology and acts as acell type-selective fluorescent marker of astrocytes bothin vivoand in slice preparations.
Solvent and concentration: DMSO: 10 mM
The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.
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- CAS. Nr.: 60311-02-6
- Formel: C31H30N2O7S2
- Molecular Weight:606.71
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biologische Aktivität
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Chemical Information
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Help & FAQs
Biologische Aktivität
Solvent and concentration: DMSO: 10 mM
The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.
Guidelines (The following is our recommended protocol. This protocol is provided for reference only and should be modified according to your specific needs).
Description: Texas Red (Sulforhodamine 101) can be used as an astrocyte marker for hippocampal slices staining with red fluorescence[3].
Method: For cell staining.
1. Stain hippocampal slices with Texas Red (5 μM; 35 min; 34°C) in carbogenated Artificial CerebroSpinal Fluid (ACSF).
2. Return hippocampal slices to dye-free ACSF for at least 30 min before imaging.
3. Use a confocal laser scanning microscopy (Zeiss LSM 880) for image.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 60311-02-6
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Molecular Weight 606.71
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Formel C31H30N2O7S2
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SMILES
O=S(C1=CC=C(C(S(=O)([O-])=O)=C1)C2=C(C=C3CCCN4CCCC5=C43)C5=[O+]C6=C7CCCN(CCC8)C7=C8C=C26)(O)=O
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Reinheit & Dokumentation
Verweise
[1]. Axel Nimmerjahn, et al. Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo. Nat Methods. 2004 Oct;1(1):31-7. [Content Brief]
[2]. J Kang, et al. Sulforhodamine 101 induces long-term potentiation of intrinsic excitability and synaptic efficacy in hippocampal CA1 pyramidal neurons. Neuroscience. 2010 Sep 15;169(4):1601-9. [Content Brief]
[3]. Wang T, et al. Malfunction of astrocyte and cholinergic input is involved in postoperative impairment of hippocampal synaptic plasticity and cognitive function. Neuropharmacology. 2022 Oct 1;217:109191. [Content Brief]
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
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