4-Di-10-ASP
Based on 1 Customer Validation
4-Di-10-ASP is a fluorescent lipophilic tracer (Excitation 485 nm; Emission 620 nm). 4-Di-10-ASP can be used to stain phospholipid membranes in a specific manner.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 95378-73-7
- Formula: C34H55IN2
- Molecular Weight:618.72
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Dissolve 4?Di?10?ASP (1 μM), DOPC (HY?113424A) (10 mM) and DOPG (HY?142980) (1 mM) in a methanol/chloroform mixture (1:2 v/v).
2. Dry the solution under vacuum overnight to obtain lamellar lipid films, which are then hydrated with transcription/translation solution at 37 °C for 3 hours.
3. Place the prepared solution on a glass slide and seal it with a coverslip.
4. Observe the sample immediately under a confocal laser-scanning microscope, and excite 4?Di?10?ASP using an argon laser (488 nm)[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
607
485
Chemical Information
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CAS No. 95378-73-7
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Appearance Solid
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Molecular Weight 618.72
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Formula C34H55IN2
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Color Orange to red
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SMILES
C[N+]1=CC=C(/C=C/C2=CC=C(N(CCCCCCCCCC)CCCCCCCCCC)C=C2)C=C1.[I-]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : 33.33 mg/mL (53.87 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (268 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Z J Huang, et al. Partition coefficients of fluorescent probes with phospholipid membranes. Biochem Biophys Res Commun. 1991 Nov 27;181(1):166-71. [Content Brief]
[2]. Shin-ichiro M Nomura, et al. Gene expression within cell-sized lipid vesicles. Chembiochem. 2003 Nov 7;4(11):1172-5. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.6162 mL | 8.0812 mL | 16.1624 mL | 40.4060 mL |
| 5 mM | 0.3232 mL | 1.6162 mL | 3.2325 mL | 8.0812 mL | |
| 10 mM | 0.1616 mL | 0.8081 mL | 1.6162 mL | 4.0406 mL | |
| 15 mM | 0.1077 mL | 0.5387 mL | 1.0775 mL | 2.6937 mL | |
| 20 mM | 0.0808 mL | 0.4041 mL | 0.8081 mL | 2.0203 mL | |
| 25 mM | 0.0646 mL | 0.3232 mL | 0.6465 mL | 1.6162 mL | |
| 30 mM | 0.0539 mL | 0.2694 mL | 0.5387 mL | 1.3469 mL | |
| 40 mM | 0.0404 mL | 0.2020 mL | 0.4041 mL | 1.0102 mL | |
| 50 mM | 0.0323 mL | 0.1616 mL | 0.3232 mL | 0.8081 mL |
4-Di-10-ASP Related Classifications
HY-D1630 Related Classifications
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