Protocol for generation and assessment of head-like structure in zebrafish

  • STAR Protoc. 2023 Sep 19;4(4):102553. doi: 10.1016/j.xpro.2023.102553.
Tao Cheng  1 ,  Yan-Yi Xing  2 ,  Yang Dong  1 ,  Peng-Fei Xu  3
Affiliations
  • 1. Women's Hospital, and Institute of Genetics, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
  • 2. Women's Hospital, and Institute of Genetics, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China; Zhejiang Provincial Key Laboratory of Genetic & Developmental Disorders, Hangzhou, Zhejiang, China.
  • 3. Women's Hospital, and Institute of Genetics, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China. Electronic address: [email protected].
PMID: 37729057 DOI: 10.1016/j.xpro.2023.102553
Abstract

In vitro embryonic analogue models, such as gastruloids, trunk-like structures and embryoids, have been developed to understand principles of early development and morphogenesis. However, models that can fully mimic head formation are still missing. Here, we present a protocol for generating the head-like structure (HLS) in zebrafish embryonic explants. We describe steps for dissection and constructing cell and patterning landscapes. We then detail assessment of this structure through axis induction. For complete details on the use and execution of this protocol, please refer to Cheng et al. (2023).1.

Keywords
Developmental Biology; Microscopy; RNAseq; Stem Cells.
Products