DHX9-IN-1
Based on 1 publication(s) in Google Scholar
DHX9-IN-1 is an inhibitor of ATP-dependent RNA helicase A (DHX9). Its EC50 value is 1.41 μM. DHX9-IN-1 has anti-tumor activity and can be used in colorectal cancer research.
For research use only. We do not sell to patients.
- Purity: 98.33%
- CAS No.: 2973747-89-4
- Formula: C21H21F2N5O3S
- Molecular Weight:461.48
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) DHX9-IN-1
MoreAll DNA/RNA Synthesis Isoforms
More
Biological Activity
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Helicase |
DHX9-IN-1 (Example 160) inhibits the proliferation of LS411N and H747 cells, with IC50 values of 9.45 and > 10 μM respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2973747-89-4
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Appearance Solid
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Molecular Weight 461.48
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Formula C21H21F2N5O3S
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Color Off-white to light yellow
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SMILES
O=C(C1=CN(C)C(C2=NC=C(N3CC(F)(F)C3)C=C2)=C1)NC4=CC=CC(NS(C)(=O)=O)=C4
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
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Nucleic Acids Res
DHX9 helicase impacts on splicing decisions by modulating U2 snRNP recruitment in Ewing sarcoma cells. [Abstract]2025 Feb 8;53(4):gkaf068. PMID: 39950342
Solvent & Solubility
DMSO : 100 mg/mL (216.69 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 3.85 mg/mL (8.34 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 3.85 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (38.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1669 mL | 10.8347 mL | 21.6694 mL | 54.1735 mL |
| 5 mM | 0.4334 mL | 2.1669 mL | 4.3339 mL | 10.8347 mL | |
| 10 mM | 0.2167 mL | 1.0835 mL | 2.1669 mL | 5.4174 mL | |
| 15 mM | 0.1445 mL | 0.7223 mL | 1.4446 mL | 3.6116 mL | |
| 20 mM | 0.1083 mL | 0.5417 mL | 1.0835 mL | 2.7087 mL | |
| 25 mM | 0.0867 mL | 0.4334 mL | 0.8668 mL | 2.1669 mL | |
| 30 mM | 0.0722 mL | 0.3612 mL | 0.7223 mL | 1.8058 mL | |
| 40 mM | 0.0542 mL | 0.2709 mL | 0.5417 mL | 1.3543 mL | |
| 50 mM | 0.0433 mL | 0.2167 mL | 0.4334 mL | 1.0835 mL | |
| 60 mM | 0.0361 mL | 0.1806 mL | 0.3612 mL | 0.9029 mL | |
| 80 mM | 0.0271 mL | 0.1354 mL | 0.2709 mL | 0.6772 mL | |
| 100 mM | 0.0217 mL | 0.1083 mL | 0.2167 mL | 0.5417 mL |
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