Thymidine  (Synonyms: DThyd; NSC 21548)

Thymidine, a specific precursor of deoxyribonucleic acid, is used as a cell synchronizing agent. Thymidine is a DNA synthesis inhibitor that can arrest cell at G1/S boundary, prior to DNA replication^[1][2][3].

Thymidine (500 mg/kg; i.p.; twice a day) completely reverses both Methotrexate- and Tomudex-induced deletion of both CD4⁺Vβ8⁺ and CD8⁺Vβ8⁺ T cells ^[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

242.23

Solid

C₁₀H₁₄N₂O₅

50-89-5

O[[email protected]]1C[[email protected]](N2C(NC(C(C)=C2)=O)=O)O[[email protected]@H]1CO

Room temperature in continental US; may vary elsewhere.

• [1]. Chen G, et al. Cell Synchronization by Double Thymidine Block. Bio Protoc. 2018 Sep 5;8(17).  [Content Brief]

  [2]. FIRKET H, et al. Autoradiographic visualization of synthesis of deoxyribonucleic acid in tissue culture with tritium-labelled thymidine. Nature. 1958 Jan 24;181(4604):274-5.FIRKET H, et al. Autoradiographic visualization of synthesis of deoxyribonucleic acid in tissue culture with tritium-labelled thymidine. Nature. 1958 Jan 24;181(4604):274-5.  [Content Brief]

  [3]. Izeradjene K, et al. Inhibition of thymidine synthesis by folate analogues induces a Fas-Fas ligand-independentdeletion of superantigen-reactive peripheral T cells. Int Immunol. 2001 Jan;13(1):85-93.  [Content Brief]