1. Cell Cycle/DNA Damage
    Metabolic Enzyme/Protease
  2. DNA/RNA Synthesis
    Endogenous Metabolite
  3. Thymidine

Thymidine  (Synonyms: DThyd; NSC 21548)

Cat. No.: HY-N1150 Purity: 99.96%
COA Handling Instructions

Thymidine, a specific precursor of deoxyribonucleic acid, is used as a cell synchronizing agent. Thymidine is a DNA synthesis inhibitor that can arrest cell at G1/S boundary, prior to DNA replication.

For research use only. We do not sell to patients.

Thymidine Chemical Structure

Thymidine Chemical Structure

CAS No. : 50-89-5

Size Price Stock Quantity
Free Sample (0.1 - 0.5 mg)   Apply Now  
10 mM * 1 mL in DMSO USD 61 In-stock
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 61 In-stock
500 mg USD 55 In-stock
1 g USD 66 In-stock
5 g   Get quote  
10 g   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 5 publication(s) in Google Scholar

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review


Thymidine, a specific precursor of deoxyribonucleic acid, is used as a cell synchronizing agent. Thymidine is a DNA synthesis inhibitor that can arrest cell at G1/S boundary, prior to DNA replication[1][2][3].

IC50 & Target[2]

Microbial Metabolite


DNA Synthesis


Human Endogenous Metabolite


In Vivo

Thymidine (500 mg/kg; i.p.; twice a day) completely reverses both Methotrexate- and Tomudex-induced deletion of both CD4+Vβ8+ and CD8+Vβ8+ T cells [3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 8-12 weeks BALB/c mice[3]
Dosage: 500 mg/kg
Administration: i.p.; twice a day
Result: Completely abrogates Methotrexate- and Tomudex-induced deletion of Vβ8+ T cells after SEB injection.
Clinical Trial
Molecular Weight







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Structure Classification

Room temperature in continental US; may vary elsewhere.

Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (206.42 mM; Need ultrasonic)

H2O : 33.33 mg/mL (137.60 mM; Need ultrasonic)

Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.1283 mL 20.6415 mL 41.2831 mL
5 mM 0.8257 mL 4.1283 mL 8.2566 mL
10 mM 0.4128 mL 2.0642 mL 4.1283 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  PBS

    Solubility: 20 mg/mL (82.57 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: 99.96%

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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