1. Cell Cycle/DNA Damage
    Metabolic Enzyme/Protease
  2. DNA/RNA Synthesis
    Endogenous Metabolite
  3. Thymidine

Thymidine (Synonyms: DThyd; NSC 21548)

Cat. No.: HY-N1150 Purity: 99.90%
Handling Instructions

Thymidine, a specific precursor of deoxyribonucleic acid, is used as a cell synchronizing agent. Thymidine is a DNA synthesis inhibitor that can arrest cell at G1/S boundary, prior to DNA replication.

For research use only. We do not sell to patients.

Thymidine Chemical Structure

Thymidine Chemical Structure

CAS No. : 50-89-5

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
500 mg USD 50 In-stock
Estimated Time of Arrival: December 31
1 g USD 60 In-stock
Estimated Time of Arrival: December 31
5 g   Get quote  
10 g   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

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Publications Citing Use of MCE Thymidine

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Description

Thymidine, a specific precursor of deoxyribonucleic acid, is used as a cell synchronizing agent. Thymidine is a DNA synthesis inhibitor that can arrest cell at G1/S boundary, prior to DNA replication[1][2][3].

IC50 & Target[2]

DNA Synthesis

 

Human Endogenous Metabolite

 

In Vivo

Thymidine (500 mg/kg; i.p.; twice a day) completely reverses both Methotrexate- and Tomudex-induced deletion of both CD4+Vβ8+ and CD8+Vβ8+ T cells [3].

Animal Model: 8-12 weeks BALB/c mice[3]
Dosage: 500 mg/kg
Administration: i.p.; twice a day
Result: Completely abrogates Methotrexate- and Tomudex-induced deletion of Vβ8+ T cells after SEB injection.
Clinical Trial
Molecular Weight

242.23

Formula

C₁₀H₁₄N₂O₅

CAS No.

50-89-5

SMILES

OC[[email protected]@H]1[[email protected]](C[[email protected]](N2C(NC(C(C)=C2)=O)=O)O1)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (206.42 mM; Need ultrasonic)

H2O : 33.33 mg/mL (137.60 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.1283 mL 20.6415 mL 41.2831 mL
5 mM 0.8257 mL 4.1283 mL 8.2566 mL
10 mM 0.4128 mL 2.0642 mL 4.1283 mL
*Please refer to the solubility information to select the appropriate solvent.
References

Purity: 99.90%

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  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

ThymidineDThydNSC 21548NSC21548NSC-21548DNA/RNA SynthesisEndogenous MetaboliteprecursorsynchronizingarrestDNAreplicationInhibitorinhibitorinhibit

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Thymidine
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HY-N1150
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