Thymidine-13C10,15N2
Thymidine-13C10,15N2 is the 13C and 15N labeled Thymidine[1]. Thymidine, a specific precursor of deoxyribonucleic acid, is used as a cell synchronizing agent. Thymidine is a DNA synthesis inhibitor that can arrest cell at G1/S boundary, prior to DNA replication[2][3][4].
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- Reinheit : 99.97%
- CAS. Nr.: 1821188-00-4
- Formel: 13C10H1415N2O5
- Molecular Weight:254.14
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Speicherung:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
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Biologische Aktivität
Beschreibung
In Vitro
Stable heavy isotopes of hydrogen, carbon, and other elements have been incorporated into drug molecules, largely as tracers for quantitation during the drug development process. Deuteration has gained attention because of its potential to affect the pharmacokinetic and metabolic profiles of drugs[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Anwendung
1. This compound can be used as a tracer
2. This compound can be used as an internal standard for quantitative analysis by NMR, GC-MS, or LC-MS.
Chemical Information
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CAS. Nr. 1821188-00-4
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Unlabeled CAS 50-89-5
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Appearance Solid
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Molecular Weight 254.14
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Formel 13C10H1415N2O5
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Color White to off-white
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SMILES
O=[13C]([15N]([13C@H](O[13C@@H]1[13CH2]O)[13CH2][13C@@H]1O)[13CH]=[13C]2[13CH3])[15NH][13C]2=O
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Synonyms
DThyd-13C10,15N2; NSC-13C10,15N2
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 50 mg/mL (196.74 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
Reinheit & Dokumentation
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Data Sheet (284 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Russak EM, et al. Impact of Deuterium Substitution on the Pharmacokinetics of Pharmaceuticals. Ann Pharmacother. 2019 Feb;53(2):211-216. [Content Brief]
[2]. Chen G, et al. Cell Synchronization by Double Thymidine Block. Bio Protoc. 2018 Sep 5;8(17). [Content Brief]
[3]. FIRKET H, et al. Autoradiographic visualization of synthesis of deoxyribonucleic acid in tissue culture with tritium-labelled thymidine. Nature. 1958 Jan 24181(4604):274-5. [Content Brief]
[4]. FIRKET H, et al. Autoradiographic visualization of synthesis of deoxyribonucleic acid in tissue culture with tritium-labelled thymidine. Nature. 1958 Jan 24 [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.9348 mL | 19.6742 mL | 39.3484 mL | 98.3710 mL |
| 5 mM | 0.7870 mL | 3.9348 mL | 7.8697 mL | 19.6742 mL | |
| 10 mM | 0.3935 mL | 1.9674 mL | 3.9348 mL | 9.8371 mL | |
| 15 mM | 0.2623 mL | 1.3116 mL | 2.6232 mL | 6.5581 mL | |
| 20 mM | 0.1967 mL | 0.9837 mL | 1.9674 mL | 4.9185 mL | |
| 25 mM | 0.1574 mL | 0.7870 mL | 1.5739 mL | 3.9348 mL | |
| 30 mM | 0.1312 mL | 0.6558 mL | 1.3116 mL | 3.2790 mL | |
| 40 mM | 0.0984 mL | 0.4919 mL | 0.9837 mL | 2.4593 mL | |
| 50 mM | 0.0787 mL | 0.3935 mL | 0.7870 mL | 1.9674 mL | |
| 60 mM | 0.0656 mL | 0.3279 mL | 0.6558 mL | 1.6395 mL | |
| 80 mM | 0.0492 mL | 0.2459 mL | 0.4919 mL | 1.2296 mL | |
| 100 mM | 0.0393 mL | 0.1967 mL | 0.3935 mL | 0.9837 mL |
Thymidine-13C10,15N2 Related Classifications
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