Thymidine-15N2
Based on 1 Customer Validation
Thymidine-15N2 is the 15N labeled Thymidine. Thymidine, a specific precursor of deoxyribonucleic acid, is used as a cell synchronizing agent. Thymidine is a DNA synthesis inhibitor that can arrest cell at G1/S boundary, prior to DNA replication.
For research use only. We do not sell to patients.
- Purity: 99.0%
- Formula: C10H1415N2O5
- Molecular Weight:244.22
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Stable heavy isotopes of hydrogen, carbon, and other elements have been incorporated into drug molecules, largely as tracers for quantitation during the drug development process. Deuteration has gained attention because of its potential to affect the pharmacokinetic and metabolic profiles of drugs[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
1. This compound can be used as a tracer
2. This compound can be used as an internal standard for quantitative analysis by NMR, GC-MS, or LC-MS.
Chemical Information
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Unlabeled Cas 50-89-5
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Appearance Solid
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Molecular Weight 244.22
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Formula C10H1415N2O5
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Color White to off-white
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SMILES
O=C1[15N](C=C(C([15NH]1)=O)C)[C@]2([H])O[C@@H]([C@H](C2)O)CO
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Synonyms
DThyd-15N2; NSC-15N2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : 50 mg/mL (204.73 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Russak EM, et al. Impact of Deuterium Substitution on the Pharmacokinetics of Pharmaceuticals. Ann Pharmacother. 2019 Feb;53(2):211-216. [Content Brief]
[2]. Chen G, et al. Cell Synchronization by Double Thymidine Block. Bio Protoc. 2018 Sep 5;8(17).;FIRKET H, et al. Autoradiographic visualization of synthesis of deoxyribonucleic acid in tissue culture with tritium-labelled thymidine. Nature. 1958 Jan 24;181(4604):274-5. [Content Brief]
[3]. FIRKET H, et al. Autoradiographic visualization of synthesis of deoxyribonucleic acid in tissue culture with tritium-labelled thymidine. Nature. 1958 Jan 24;181(4604):274-5. [Content Brief]
[4]. Izeradjene K, et al. Inhibition of thymidine synthesis by folate analogues induces a Fas-Fas ligand-independentdeletion of superantigen-reactive peripheral T cells. Int Immunol. 2001 Jan13(1):85-93. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.0947 mL | 20.4733 mL | 40.9467 mL | 102.3667 mL |
| 5 mM | 0.8189 mL | 4.0947 mL | 8.1893 mL | 20.4733 mL | |
| 10 mM | 0.4095 mL | 2.0473 mL | 4.0947 mL | 10.2367 mL | |
| 15 mM | 0.2730 mL | 1.3649 mL | 2.7298 mL | 6.8244 mL | |
| 20 mM | 0.2047 mL | 1.0237 mL | 2.0473 mL | 5.1183 mL | |
| 25 mM | 0.1638 mL | 0.8189 mL | 1.6379 mL | 4.0947 mL | |
| 30 mM | 0.1365 mL | 0.6824 mL | 1.3649 mL | 3.4122 mL | |
| 40 mM | 0.1024 mL | 0.5118 mL | 1.0237 mL | 2.5592 mL | |
| 50 mM | 0.0819 mL | 0.4095 mL | 0.8189 mL | 2.0473 mL | |
| 60 mM | 0.0682 mL | 0.3412 mL | 0.6824 mL | 1.7061 mL | |
| 80 mM | 0.0512 mL | 0.2559 mL | 0.5118 mL | 1.2796 mL | |
| 100 mM | 0.0409 mL | 0.2047 mL | 0.4095 mL | 1.0237 mL |
Thymidine-15N2 Related Classifications
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