Thymidine-13C5
Based on 1 Customer Validation
Thymidine-13C5 (DThyd-13C5; NSC 21548-13C5) is 13C-labeled Thymidine (HY-N1150).
For research use only. We do not sell to patients.
- Purity: 99.90%
- CAS No.: 156968-81-9
- Formula: C513C5H14N2O5
- Molecular Weight:247.19
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Storage:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Stable or radioisotope-labeled compounds allow precise tracking and quantification of individual atoms in metabolic pathways. Stable isotopes generally do not change molecular properties but may slightly affect metabolic kinetics; radioactive isotopes may interfere with cells. Markers can distinguish endogenous and exogenous metabolites, reduce false positives, and are beneficial to quantification and reconstruction of metabolic pathways[2].
In cell culture or enzymatic reactions, the use of isotope markers can precisely control the concentration and exposure time, making it easy to study metabolic reactions and enzyme activities. Through stable isotope analytical metabolomics (SIRM), cellular metabolic networks can be studied, key metabolic nodes and regulatory mechanisms can be identified, and targets can be provided for compound development.
Isotope-labeled compounds can be used in competition binding experiments to evaluate the affinity and binding kinetics of compounds to receptors to help optimize design. Stable isotope labels are used as internal standards in mass spectrometry analysis to improve analysis accuracy and reproducibility and reduce matrix effect interference[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Isotope labeling can reveal specific steps in metabolic pathways. Using compounds with stable isotope labels at specific locations directly in humans or animal models can also help verify drug mechanisms and evaluate unexpected side effects, improving the accuracy and efficiency of clinical research[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
1. This compound can be used as a tracer
2. This compound can be used as an internal standard for quantitative analysis by NMR, GC-MS, or LC-MS.
Chemical Information
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CAS No. 156968-81-9
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Unlabeled Cas 50-89-5
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Appearance Solid
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Molecular Weight 247.19
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Formula C513C5H14N2O5
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Color White to off-white
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SMILES
O=C1N(C=C(C(N1)=O)C)[13C@@H]2O[13C@@H]([13C@H]([13CH2]2)O)[13CH2]O
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Synonyms
DThyd-13C5; NSC 21548-13C5
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
DMSO : 50 mg/mL (202.27 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (276 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Russak EM, et al. Impact of Deuterium Substitution on the Pharmacokinetics of Pharmaceuticals. Ann Pharmacother. 2019;53(2):211-216. [Content Brief]
[2]. Smith K A, et al. Soil and environmental analysis[M]. Marcel Dekker Incorporated, 2000.
[3]. Fan T W M, et al. Stable isotope-resolved metabolomics and applications for drug development[J]. Pharmacology & therapeutics, 2012, 133(3): 366-391. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.0455 mL | 20.2274 mL | 40.4547 mL | 101.1368 mL |
| 5 mM | 0.8091 mL | 4.0455 mL | 8.0909 mL | 20.2274 mL | |
| 10 mM | 0.4045 mL | 2.0227 mL | 4.0455 mL | 10.1137 mL | |
| 15 mM | 0.2697 mL | 1.3485 mL | 2.6970 mL | 6.7425 mL | |
| 20 mM | 0.2023 mL | 1.0114 mL | 2.0227 mL | 5.0568 mL | |
| 25 mM | 0.1618 mL | 0.8091 mL | 1.6182 mL | 4.0455 mL | |
| 30 mM | 0.1348 mL | 0.6742 mL | 1.3485 mL | 3.3712 mL | |
| 40 mM | 0.1011 mL | 0.5057 mL | 1.0114 mL | 2.5284 mL | |
| 50 mM | 0.0809 mL | 0.4045 mL | 0.8091 mL | 2.0227 mL | |
| 60 mM | 0.0674 mL | 0.3371 mL | 0.6742 mL | 1.6856 mL | |
| 80 mM | 0.0506 mL | 0.2528 mL | 0.5057 mL | 1.2642 mL | |
| 100 mM | 0.0405 mL | 0.2023 mL | 0.4045 mL | 1.0114 mL |
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