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  3. Anisomycin

Anisomycin (Synonyms: Flagecidin; Wuningmeisu C)

Cat. No.: HY-18982 Purity: 98.27%
Handling Instructions

Anisomycin is a potent protein synthesis inhibitor which interferes with protein and DNA synthesis by inhibiting peptidyl transferase or the 80S ribosome system. Anisomycin is a JNK activator, which increases phospho-JNK. Anisomycin is a bacterial antibiotic.

For research use only. We do not sell to patients.

Anisomycin Chemical Structure

Anisomycin Chemical Structure

CAS No. : 22862-76-6

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Solution
10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
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10 mM * 1 mL
ready for reconstitution
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Estimated Time of Arrival: December 31
Solid
10 mg USD 60 In-stock
Estimated Time of Arrival: December 31
50 mg USD 120 In-stock
Estimated Time of Arrival: December 31
100 mg USD 180 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 35 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Anisomycin purchased from MCE. Usage Cited in: Chem Biol Interact. 2017 Nov 1;277:62-73.

    Effects of silencing p53 on colistin-induced ROS production and p-JNK expression level in PC-12 cells. The protein levels of p53. The protein levels of p-JNK by western blot.

    Anisomycin purchased from MCE. Usage Cited in: Biochem Biophys Res Commun. 2018 May 23;499(4):743-750.

    Astrocytes are cultured with p38 and JNK activator of DHC and ANS, respectively, at the indicated concentrations for 24 h. Then, all cells are harvested for western blot analysis of p-p38 and p-JNK. Astrocytes are pretreated with DHC (500 nM) or ANS (10 mM) for 24 h, followed by Fru (5 mM) for an additional 24 h.

    Anisomycin purchased from MCE. Usage Cited in: Sci Rep. 2018 Apr 23;8(1):6379.

    Immunoblot analysis of lysates from Anisomycin pre-treatment cells reveals an increase of CHIP protein compared with OGD treated only cells, contrasting with a decrease in RIPK3 and p-MLKL protei. Pre-treatment cells with SP600125 results in the expected attenuation in JNK phosphorylation levels.

    Anisomycin purchased from MCE. Usage Cited in: Am J Physiol Cell Physiol. 2018 Aug 1;315(2):C225-C235.

    Western blot analysis shows that inhibiting MAPK cannot completely reverse increased expression of RNF2 and CDDP resistance of OC cells.

    Anisomycin purchased from MCE. Usage Cited in: Biochem Biophys Res Commun. 2018 Sep 5;503(2):467-473.

    The increase of Bcl-2 and decrease of Bax, cleaved Caspase-3 and PARP induced by Ulk1- inhibition in LPS-exposed AST are abrogated by the Dehydrocorydaline chloride (DHC) or Anisomycin (ANS) pre-treatment

    Anisomycin purchased from MCE. Usage Cited in: Eur J Pharmacol. 2018 Sep 5;834:295-304.

    The protein levels of p-JNK by western blot following Anisomycin (4 μM) treatment for 24 h after Morroniside (100 μM) 12 h.

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    Description

    Anisomycin is a potent protein synthesis inhibitor which interferes with protein and DNA synthesis by inhibiting peptidyl transferase or the 80S ribosome system[1]. Anisomycin is a JNK activator, which increases phospho-JNK[2][3]. Anisomycin is a bacterial antibiotic[4].

    IC50 & Target[1][3]

    JNK

     

    DNA synthesis

     

    In Vitro

    To examine whether JNK has a core role in colistin-induced neurotoxicity in PC-12 cells, an SP600125 (a highly selective inhibitor of JNK) and Anisomycin (a potent activator) are used in this study. In order to select an appropriate concentration, PC-12 cells are treated with a range of SP600125 (0-80 μM) and Anisomycin (0-20 μM) respectively for 24 h. The results show that the cells viability significantly decreases by SP600125 treatment in a concentration-dependent manner, observed at the concentrations greater than 20 μM (p<0.01). Similarly the cells viability is inhibited by Anisomycin treatment (≥8 μM) (p<0.05) [1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    In Vivo

    Anisomycin (60 mg/kg; for 4-week continuous intravenous administration) significantly decreases mouse body weight in a dose-related manner, compared with the control group. Anisomycin (15 mg/kg; for 4-week continuous intravenous administration) slightly and transiently decreases the mouse body weight. There is no significant difference of the mouse body weight in 5 mg/kg group[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Balb/c mice of both sexes (4-5 weeks old)[3]
    Dosage: 84, 99, 116, 136 or 160 mg/kg; 0.2 mL per mouse
    Administration: Intravenously injected through mouse tail vein
    Result: The calculated LD50 for Anisomycin was 119.64 mg/kg.
    Molecular Weight

    265.31

    Formula

    C₁₄H₁₉NO₄

    CAS No.
    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 50 mg/mL (188.46 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.7692 mL 18.8459 mL 37.6918 mL
    5 mM 0.7538 mL 3.7692 mL 7.5384 mL
    10 mM 0.3769 mL 1.8846 mL 3.7692 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (9.42 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.5 mg/mL (9.42 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (9.42 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References

    Purity: 98.27%

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    Product Name:
    Anisomycin
    Cat. No.:
    HY-18982
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