1. Cell Cycle/DNA Damage
    Stem Cell/Wnt
    JAK/STAT Signaling
    Apoptosis
  2. Nucleoside Antimetabolite/Analog
    DNA/RNA Synthesis
    STAT
    Apoptosis
  3. Fludarabine

Fludarabine (Synonyms: F-ara-A; NSC 118218)

Cat. No.: HY-B0069 Purity: 99.85%
Handling Instructions

Fludarabine (NSC 118218) is a DNA synthesis inhibitor and a fluorinated purine analogue with antineoplastic activity in lymphoproliferative malignancies. Fludarabine inhibits the cytokine-induced activation of STAT1 and STAT1-dependent gene transcription in normal resting or activated lymphocytes.

For research use only. We do not sell to patients.

Fludarabine Chemical Structure

Fludarabine Chemical Structure

CAS No. : 21679-14-1

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Solution
10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 66 In-stock
Estimated Time of Arrival: December 31
Solid
5 mg USD 60 In-stock
Estimated Time of Arrival: December 31
10 mg USD 72 In-stock
Estimated Time of Arrival: December 31
50 mg USD 144 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 18 publication(s) in Google Scholar

Other Forms of Fludarabine:

Top Publications Citing Use of Products

    Fludarabine purchased from MCE. Usage Cited in: Nat Commun. 2021 Mar 29;12(1):1940.

    SW1990 cells transfected with indicated 20, 50 μM Fludara with 20 ng/mL IFN-γ for 24 hours, immunoblotting analyses are performed using the indicated antibodies.

    Fludarabine purchased from MCE. Usage Cited in: J Exp Clin Cancer Res. 2019 Aug 22;38(1):370.

    Western blot of JAK2 and STAT1 phosphorylation and CXCL1 expression in cells pretreated with Fludarabine (100 μM) for 1 h and subsequently treated with VP-16 (20 μM) or CPT-11 (80 μg/mL) for 0.5 h.

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    Description

    Fludarabine (NSC 118218) is a DNA synthesis inhibitor and a fluorinated purine analogue with antineoplastic activity in lymphoproliferative malignancies. Fludarabine inhibits the cytokine-induced activation of STAT1 and STAT1-dependent gene transcription in normal resting or activated lymphocytes[1][2][3][4].

    In Vitro

    Fludarabine (5 μM; 48 hours) induces a decrease in p27kip1 expression[4].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Western Blot Analysis[4]

    Cell Line: B-CLL cells
    Concentration: 5 μM
    Incubation Time: 24 hours
    Result: Induces a decrease in p27kip1 expression. The decrease in p27kip1 expression was significantly correlated to the extent of in vitro apoptosis.
    In Vivo

    Fludarabine (0.8 mg/kg; i.p.; two cycles for 5 days every 2 weeks) in combination with Cyclophosphamide (400 mg/kg; i.p.; 2 weeks) decreases incidence of GVHD[5].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: F-1 mice received 105 BCL-1 leukemia cells[5]
    Dosage: 0.8 mg/kg
    Administration: Intraperitoneal injection; two cycles for 5 days every 2 weeks
    Result: Combination with Cyclophosphamide decreased incidence of graft-versus-host disease (GVHD).
    Clinical Trial
    Molecular Weight

    285.23

    Formula

    C₁₀H₁₂FN₅O₄

    CAS No.
    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 25 mg/mL (87.65 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.5059 mL 17.5297 mL 35.0594 mL
    5 mM 0.7012 mL 3.5059 mL 7.0119 mL
    10 mM 0.3506 mL 1.7530 mL 3.5059 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (8.76 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.5 mg/mL (8.76 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (8.76 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
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    Product Name:
    Fludarabine
    Cat. No.:
    HY-B0069
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