1. Cell Cycle/DNA Damage
  2. Nucleoside Antimetabolite/Analog

5-BrdU (Synonyms: BUdR; BRDU)

Cat. No.: HY-15910 Purity: 99.99%
Handling Instructions

5-BrdU is a nucleoside analog that competes with thymidine for incorporation into DNA. 5-BrdU is commonly used in the detection of proliferating cells.

For research use only. We do not sell to patients.

5-BrdU Chemical Structure

5-BrdU Chemical Structure

CAS No. : 59-14-3

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Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
100 mg USD 60 In-stock
Estimated Time of Arrival: December 31
500 mg USD 84 In-stock
Estimated Time of Arrival: December 31
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Customer Review

    5-BrdU purchased from MCE. Usage Cited in: Obesity (Silver Spring). 2017 Sep;25(9):1532-1539.

    Representative images for BrdU staining, followed by semiquantitative analysis.

    5-BrdU purchased from MCE. Usage Cited in: Am J Transl Res. 2016 Jun 15;8(6):2562-74.

    BrdU hepatocyte proliferative assay in ESLC. The representative tissue containing BrdU-positive cells taken after 6 hours of ESLC is shown in (A), and two newly proliferated neighboring positive cells is enlarged and inserted on the right side. (B) shows the experimental negative control and (C) is the analyzed number of BrdU-positive hepatocytes in comparison with control.

    5-BrdU purchased from MCE. Usage Cited in: Exp Ther Med. 2018 Jun;15(6):5269-5274.

    Detection of the effects of Letrozole and the MAPK pathway on the proliferation of GC-1 spg cells by BrdU. MAPK, mitogen-activated protein kinase; spg, spermatogonia; BrdU, bromodeoxyuridine.

    5-BrdU purchased from MCE. Usage Cited in: Exp Ther Med. 2018 Jun;15(6):5269-5274.

    Letrozole significantly increases the protein levels of Ras/ERK1/c-Myc in the classical MAPK pathway.
    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References

    Description

    5-BrdU is a nucleoside analog that competes with thymidine for incorporation into DNA. 5-BrdU is commonly used in the detection of proliferating cells.

    In Vitro

    Bromodeoxyuridine induces a progressive, dose-responsive suppression of cancer cell line and cancer stem cell population expansion RG2 rat glioma cells. In H9 cells and BJ fibroblasts, bromodeoxyuridine alters the cell cycle profile[1]. BrdU is stably integrated into the DNA, and thus can be used in assessment of cell proliferation and other cell procession[2]

    In Vivo

    Bromodeoxyuridine (300 mg/kg, i.p. or 0.8 mg/mL, p.o.) significantly slows tumor progression in rat glioma RG2 tumor model[1].

    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 41 mg/mL (133.51 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.2563 mL 16.2813 mL 32.5627 mL
    5 mM 0.6513 mL 3.2563 mL 6.5125 mL
    10 mM 0.3256 mL 1.6281 mL 3.2563 mL
    *Please refer to the solubility information to select the appropriate solvent.
    References
    Cell Assay
    [1]

    Cultures are initially plated at 2000 cells/cm2 and are quantified with a Z2 Coulter Counter. RG2 rat glioma cells are treated once with 0, 1, 10, or 50 µM BrdU for 24 hours, and cumulative growth curves are obtained over 18 days. Control and treated cells are quantified and replated at equal densities on days 5, 12, and 18 after treatment.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    Molecular Weight

    307.10

    Formula

    C₉H₁₁BrN₂O₅

    CAS No.

    59-14-3

    SMILES

    OC[[email protected]@H]1[[email protected]](C[[email protected]](N2C(NC(C(Br)=C2)=O)=O)O1)O

    Storage

    4°C, protect from light

    Shipping

    Room temperature in continental US; may vary elsewhere

    Purity: 99.99%

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    This equation is commonly abbreviated as: C1V1 = C2V2

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    × = ×
    C1   V1   C2   V2

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    Product Name:
    5-BrdU
    Cat. No.:
    HY-15910
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    5-BrdU

    Cat. No.: HY-15910