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  4. Streptozotocin

Streptozotocin  (Synonyms: Streptozocin; NSC-85998; U 9889)

Cat. No.: HY-13753 Purity: 99.15%
COA Handling Instructions

Streptozotocin (Streptozocin) is an antibiotic widely used in experimental animal models of induced diabetes. Streptozotocin enters B cells via the glucose transporter (GLUT2) and causes the alkylation of DNA ( DNA-methylating ). Streptozotocin can induce the apoptosis of β cells.

For research use only. We do not sell to patients.

Streptozotocin Chemical Structure

Streptozotocin Chemical Structure

CAS No. : 18883-66-4

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Based on 38 publication(s) in Google Scholar

Top Publications Citing Use of Products

38 Publications Citing Use of MCE Streptozotocin

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Description

Streptozotocin (Streptozocin) is an antibiotic widely used in experimental animal models of induced diabetes. Streptozotocin enters B cells via the glucose transporter (GLUT2) and causes the alkylation of DNA ( DNA-methylating ). Streptozotocin can induce the apoptosis of β cells[1][2][3][4][5][6][7].

IC50 & Target

DNA alkylator[1]

In Vitro

The IC50 values of Streptozotocin for HL60, K562 and C1498 cells were 11.7, 904 and 1024 μg/ml, respectively[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Streptozotocin (180 mg/kg, intravenous injection, killed 4 days later) can induce diabetes mellitus and lymphocytopenia in mice[2].
Streptozotocin (STZ) is a classic diabetes modeling agent that induces disease by destroying pancreatic beta cells in animals. And rats and mice are generally used as animal models. Different injection doses of STZ induce different diabetes models (T1DM, T2DM)[3][4][5].

Dosage reference for Streptozotocin-induced diabetes model in experimental animals[3][4]
(1) T1DM
Male Wistar rats: Single high-dose injection of 50 mg/kg STZ
Female C57BL/6J mice: Single high-dose injection of 200 mg/kg STZ
(2) T2DM
Male Wistar rats: 8 weeks of high-fat diet + low-dose injection of 25 mg/kg STZ for 5 days
Female C57BL/6J mice: high-fat diet + low-dose injection of 40 mg/kg STZ for 4 days


For the dissolution method of Streptozotocin, please refer to [5]:
(1) Solvent preparation: 0.1 mM citrate buffer
Liquid A: Weigh 2.1 g of citric acid (HY-N1428) (MW: 210.14), add double distilled water to 100 mL, and dissolve
Liquid B: Sodium citrate (HY-B2201) (MW: 294.10) 2.94 g. Add double distilled water to 100 mL and dissolve
Citrate buffer: Mix solution A and solution B in a ratio of 1.32:1. Determine pH and adjust to 4.2-4.5. Finally, use a 0.22 µm or 0.45 µm filter to remove impurities.
(2) Streptozotocin working solution preparation
Use the above buffer solution to prepare Streptozotocin injection (prepare in ice bath). The injection solution should be prepared for immediate use or stored at 4°C, and the injection should be completed within 30 minutes.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 male mice[2]
Dosage: 180 mg/kg
Administration: i.v.
Result: Elevated blood glucose levels after 48 h and reduced body weight.
Inhibited splenocyte proliferation in mixed lymphocyte cultures.
Increased the level of INF-γ.
Clinical Trial
Molecular Weight

265.22

Formula

C8H15N3O7

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](NC(N(C)N=O)=O)[C@H]1O

Structure Classification
Initial Source

Streptomyces achromogenes

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture and light

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (942.61 mM; Need ultrasonic)

H2O : 113.3 mg/mL (427.19 mM; Need ultrasonic and warming)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.7705 mL 18.8523 mL 37.7045 mL
5 mM 0.7541 mL 3.7705 mL 7.5409 mL
10 mM 0.3770 mL 1.8852 mL 3.7705 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  0.1 M Sodium citrate buffer (pH 4.5)

    Solubility: 200 mg/mL (754.09 mM); Clear solution; Need ultrasonic

*All of the co-solvents are available by MedChemExpress (MCE).
Purity & Documentation

Purity: 99.15%

References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Streptozotocin
Cat. No.:
HY-13753
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