Sialidase (α2-3-6-8-9)

Product Information
This product is expressed by recombinant Escherichia coli. The enzyme can hydrolyze sialic acid connected by α2-3, α2-6, α2-8 and α2-9 bonds on glycoproteins and oligosaccharides. The rate of hydrolyzing α2-3 and α2-6 bonds is greater than that of α2-8 and α2-9 bonds.
Storage buffer: 20 mM Tris, 50 mM NaCl, 1 mM EDTA, pH 8.0

Instructions
1. According to the reaction system, add substrate (2 μg glycoprotein or 200 nM oligosaccharide), 1.0 μL of sialidase (α2-3,6,8,9), 2.5 μL of reaction buffer (50 mM CaCl₂, 500 mM sodium acetate, pH 5.5), and add water to 25 μL;
2. After mixing, incubate the sample at 37°C for 5-60 min. Heat at 65°C for 10 min to terminate the reaction.

Notes
The pH of the above-mentioned sialidase reaction buffer is 5.5. If you want to enzymatically cleave sialic acid on the cell surface, please replace the buffer system that is compatible with the cells.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.