Vaccinia virus capping enzyme
Based on 1 Customer Validation
Vaccinia virus capping enzyme is a transcription initiation factor. Vaccinia virus capping enzyme is a heterodimer of D1 (844 aa) and D12 (287 aa) polypeptides that executes all three steps in m7GpppRNA synthesis. Vaccinia virus capping enzyme has been used widely as a reagent for capping and cap-labeling RNAs in vitro.
For research use only. We do not sell to patients.
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Vaccinia virus capping enzyme is involved both in the formation of a 5′ cap structure and in termination of early transcription[1].
| Specification | Type | Components | Volume | Quantity |
| 500 U | A | Vaccinia virus capping enzyme | 500 U | 1 |
| 500 U | B | 10 × Cap Buffer | 0.1 mL | 1 |
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
10 U/μL
One unit is defined as the amount of enzyme that 10 pmol GTP to be fully incorporated into the 80 nt transcript product within 1 h at 37°C.
Chemical Information
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Appearance Liquid
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Color Colorless to light yellow
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SMILES
[Vaccinia virus capping enzyme]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (264 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Vos JC, et al. Vaccinia virus capping enzyme is a transcription initiation factor. EMBO J. 1991 Sep;10(9):2553-8. [Content Brief]
[2]. Kyrieleis OJ, et al. Crystal structure of vaccinia virus mRNA capping enzyme provides insights into the mechanism and evolution of the capping apparatus. Structure. 2014 Mar 4;22(3):452-65. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Vaccinia virus capping enzyme Related Classifications
HY-E70080 Related Classifications
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