T7 RNA polymerase
Based on 1 Customer Validation
T7 RNA polymerase is a polymerase expressed by Escherichia coli from the RNA polymerase gene of T7 bacteriophage. T7 RNA polymerase is highly specific and involved in in vitro transcription (IVT) of mRNA. In the presence of Mg2+, T7 RNA polymerase only uses the single-stranded or double-stranded DNA containing the T7 promoter sequence as a template, and uses NTP as a substrate to synthesize RNA complementary to the single-stranded DNA downstream of the promoter.
For research use only. We do not sell to patients.
- CAS No.: 9014-24-8
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Purity & Documentation
- References
-
Help & FAQs
All DNA/RNA Synthesis Isoforms
More
Biological Activity
|
RNA Polymerase |
Product information
This product is recombinantly expressed in Escherichia coli .
Storage buffer: 20 mM Tris-HCl, 100 mM NaCl, 10 mM DTT, 0.1% Triton X-100, 1 mM EDTA, 50% Glycerol, pH7.9.
Reaction buffer: 400 mM Tris-HCl, 60 mM MgCl2, 10 mM DTT, 20 mM spermidine, pH 7.9
Instructions
1. Please refer to the following reaction system
0.4 μL 25mM NTP Mix, 0.2-1 ug DNA template, 2 μL reaction buffer, 0.5 μL RNase Inhibitor (optional, 1 U/μL), 1 μL 0.1 M DTT (optional), 2 μL T7 RNA polymerase, use RNase Make up the volume to 20 μL with free water.
2.Reaction conditions
Incubate at 37°C for 0.5-1h. For fragments smaller than 300 nt, it is recommended to incubate at 37°C for 2-16 h.
Notes
1. After the reaction, use 2 U DNase I (RNase-free) at 37°C for 15 minutes to remove the DNA template.
2. Adding 0.02 U thermostable inorganic pyrophosphatase to the 20 μL reaction system can significantly increase the transcription yield.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
≥50 U/μL
One unit is defined as the amount of enzyme that catalyze the incorporation of 1 nmol AMP into the polynucleotide at 37°C in 60 min.
Chemical Information
-
CAS No. 9014-24-8
-
Appearance Liquid
-
Color Colorless to light yellow
-
SMILES
[T7 RNA polymerase]
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
-
Data Sheet (268 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
References
[1]. Dousis A, et al. An engineered T7 RNA polymerase that produces mRNA free of immunostimulatory byproducts. Nat Biotechnol. 2023 Apr;41(4):560-568. [Content Brief]
[2]. Borkotoky S, et al. The highly efficient T7 RNA polymerase: A wonder macromolecule in biological realm. Int J Biol Macromol. 2018 Oct 15;118(Pt A):49-56. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
T7 RNA polymerase Related Classifications
HY-E70090 Related Classifications
Powered by Bioz