9015-85-4
Chemical Structure
Biological Activity: T4 DNA ligase is the product of gene 30 of phage T4. T4 DNA ligase catalyzes the repair of single-stranded nicks in duplex DNA and joins duplex DNA restriction fragments having either blunt or cohesive ends. T4 DNA ligase catalyze the sealing of adjacent 5′-phosphate and 3′-hydroxyl termini at single-stranded breaks in double-stranded DNA.T4 DNA ligase is an ATP-dependent ligase enzyme. T4 DNA ligase can be used in various biotechnological applications. T4 DNA ligase can join the ends of single-stranded DNA in the absence of any duplex DNA structure at the ligation site[1][2][3].
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T4 DNA ligase | T4 DNA ligase is the product of gene 30 of phage T4. T4 DNA ligase catalyzes the repair of single-stranded nicks in duplex DNA and joins duplex DNA restriction fragments having either blunt or cohesive ends. T4 DNA ligase catalyze the sealing of adjacent 5′-phosphate and 3′-hydroxyl termini at single-stranded breaks in double-stranded DNA.T4 DNA ligase is an ATP-dependent ligase enzyme. T4 DNA ligase can be used in various biotechnological applications. T4 DNA ligase can join the ends of single-stranded DNA in the absence of any duplex DNA structure at the ligation site. | |||||||||||||||||||||
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- [1]. Kuhn, H., & Frank-Kamenetskii, M. D. (2005). Template-independent ligation of single-stranded DNA by T4 DNA ligase. The FEBS journal, 272(23), 5991-6000. [Content Brief]
- [2]. Shi, K., et al., (2018). T4 DNA ligase structure reveals a prototypical ATP-dependent ligase with a unique mode of sliding clamp interaction. Nucleic acids research, 46(19), 10474-10488. [Content Brief]
- [3]. Tabor S. DNA ligases. Curr Protoc Mol Biol. 2001 May;Chapter 3:Unit3.14. [Content Brief]
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